Preparation and characterization of everted membrane vesicles from cells of Staphylococcus aureus

被引:0
作者
Kodama, K
Hashimoto, A
Morita, Y
Tomochika, K
Tsuchiya, T [1 ]
机构
[1] Okayama Univ, Fac Pharmaceut Sci, Dept Microbiol, Okayama 700, Japan
[2] Okayama Univ, Fac Pharmaceut Sci, Dept Environm Hyg, Okayama 700, Japan
关键词
everted membrane vesicle; H+-ATPase; drug/H+ antiporter; S-aureus;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
We developed a method for the preparation of everted membrane vesicles from cells of Staphylococcus aureus. The cells were first treated with ampicillin to weaken the peptidoglycan layer, then the cells were passed through a French press cell. The resulting vesicles were roughly 0.1 mu m in diameter, judging from electron microscopic observations. We detected fairly high membrane-bound ATPase activity in the membrane vesicles. We observed respiratory-driven quenching of quinacrine fluorescence, which indicates that inward H+ transport took place. These results indicate that the vesicles are everted. We characterized the membrane-bound ATPase. We also detected Na+/H+ antiport, erythromycin/H+ antiport and chloramphenicol/H+ antiport activities in the membranes of S. aureus.
引用
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页码:5 / 9
页数:5
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