Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus

被引:15
作者
Halwe, Nico Joel [1 ]
Gorka, Marco [1 ]
Hoffmann, Bernd [1 ]
Rissmann, Melanie [2 ]
Breithaupt, Angele [3 ]
Schwemmle, Martin [4 ,5 ]
Beer, Martin [1 ]
Kandeil, Ahmed [6 ]
Ali, Mohamed A. [6 ]
Kayali, Ghazi [7 ,8 ]
Hoffmann, Donata [1 ]
Balkema-Buschmann, Anne [2 ]
机构
[1] Friedrich Loeffler Inst, Inst Diagnost Virol, D-17493 Greifswald, Insel Riems, Germany
[2] Friedrich Loeffler Inst, Inst Novel & Emerging Infect Dis, D-17493 Greifswald, Insel Riems, Germany
[3] Friedrich Loeffler Inst, Dept Expt Anim Facil & Bior Management, D-17493 Greifswald, Insel Riems, Germany
[4] Univ Freiburg, Inst Virol, Med Ctr, D-79104 Freiburg, Germany
[5] Univ Freiburg, Fac Med, D-79104 Freiburg, Germany
[6] Natl Res Ctr, Ctr Sci Excellence Influenza Viruses, Giza 12311, Egypt
[7] Univ Texas Hlth Sci Ctr Houston, Dept Epidemiol Human Genet & Environm Sci, Houston, TX 77030 USA
[8] Human Link, Dubai, U Arab Emirates
来源
VIRUSES-BASEL | 2021年 / 13卷 / 04期
关键词
influenza virus; Rousettus aegyptiacus; PATHOGENICITY; DIVERSITY; RISK;
D O I
10.3390/v13040672
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Influenza A viruses (IAV) of subtype H9N2, endemic in world-wide poultry holdings, are reported to cause spill-over infections to pigs and humans and have also contributed substantially to recent reassortment-derived pre-pandemic zoonotic viruses of concern, such as the Asian H7N9 viruses. Recently, a H9N2 bat influenza A virus was found in Egyptian fruit bats (Rousettus aegyptiacus), raising the question of whether this bat species is a suitable host for IAV. Here, we studied the susceptibility, pathogenesis and transmission of avian and bat-related H9N2 viruses in this new host. In a first experiment, we oronasally inoculated six Egyptian fruit bats with an avian-related H9N2 virus (A/layer chicken/Bangladesh/VP02-plaque/2016 (H9N2)). In a second experiment, six Egyptian fruit bats were inoculated with the newly discovered bat-related H9N2 virus (A/bat/Egypt/381OP/2017 (H9N2)). While R. aegyptiacus turned out to be refractory to an infection with H9N2 avian-type, inoculation with the bat H9N2 subtype established a productive infection in all inoculated animals with a detectable seroconversion at day 21 post-infection. In conclusion, Egyptian fruit bats are most likely not susceptible to the avian H9N2 subtype, but can be infected with fruit bat-derived H9N2. H9-specific sero-reactivities in fruit bats in the field are therefore more likely the result of contact with a bat-adapted H9N2 strain.
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页数:14
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