Interstitial exclusion of macromolecules studied by graded centrifugation of rat tail tendon

被引:21
作者
Aukland, K [1 ]
Wiig, H
Tenstad, O
机构
[1] Univ Bergen, Dept Physiol, N-5009 Bergen, Norway
[2] Univ Calif Davis, Dept Human Physiol, Davis, CA 95616 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1997年 / 273卷 / 06期
关键词
collagen fibrils; hyaluronan; proteoglycans; plasma proteins; aprotinin;
D O I
10.1152/ajpheart.1997.273.6.H2794
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Mechanical compression of cartilage and tendon has been shown to expel fluid both from collagen fibrils and from the extrafibrillar space. As reported previously, albumin (Alb) concentration and colloid osmotic pressure in tendon fluid (TF) expelled by repeated centrifugations fell progressively at increasing centrifugation force (G = 600, 2,400, and 13,100), suggesting either molecular sieving in compressed tendon or mobilization of protein-free (excluded) fluid. The present experiments, including analysis of Cr-51-EDTA, aprotinin (Ap), Alb, immunoglobulin G (IgG), and hyaluronan (hyaluronic acid; HA) with molecular weight (MW) ranging from 341 to 5 x 10(6), strongly favored the exclusion hypothesis; the fraction of Alb, IgG, and HA-free fluid (excluded) was already 0.23-0.36 in the first centrifugate, increasing to 0.73-0.82 in the third. The corresponding numbers were, respectively, 0.11 and 0.43 for Ap (MW 6,500), and 0 and 0.08 for Cr-51-EDTA. These data, combined with calculated exclusion by collagen fibrils, proteoglycans, and HA, indicated that the first centrifugate was mainly derived from the extrafibrillar space, with increasing addition of macromolecular free intrafibrillar fluid in the second and third centrifugates, with each space contributing about equally to the total centrifugate volume. The calculations also indicated that Alb-, IgG-, and Ap-free fluid was mobilized from extrafibrillar space by increasing overlap of excluded territories. An excess of HA in tendon compared with that estimated from centrifugate concentrations suggests a large bound or immobilized HA fraction.
引用
收藏
页码:H2794 / H2803
页数:10
相关论文
共 35 条
[1]   ISOLATION OF A GLYCOPROTEIN AND PROTEODERMATAN SULFATE FROM BOVINE ACHILLES-TENDON BY AFFINITY CHROMATOGRAPHY ON CONCANAVALIN A-SEPHAROSE [J].
ANDERSON, JC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 379 (02) :444-455
[2]   DISTRIBUTION VOLUMES AND MACROMOLECULAR MOBILITY IN RAT TAIL TENDON INTERSTITIUM [J].
AUKLAND, K .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02) :H409-H419
[3]   PROTEIN CONCENTRATION OF INTERSTITIAL FLUID COLLECTED FROM RAT SKIN BY A WICK METHOD [J].
AUKLAND, K ;
FADNES, HO .
ACTA PHYSIOLOGICA SCANDINAVICA, 1973, 88 (03) :350-358
[4]   THE EXCLUSION OF HUMAN-SERUM ALBUMIN BY HUMAN DERMAL COLLAGENOUS FIBERS AND WITHIN HUMAN DERMIS [J].
BERT, JL ;
MATHIESON, JM ;
PEARCE, RH .
BIOCHEMICAL JOURNAL, 1982, 201 (02) :395-403
[5]   PERSISTENCE LENGTH OF HYALURONIC-ACID - ESTIMATE FROM SMALL-ANGLE X-RAY-SCATTERING AND INTRINSIC-VISCOSITY [J].
CLELAND, RL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 180 (01) :57-68
[6]  
Curry F.-R.E., 1984, Handb. Physiol, V2, P309, DOI DOI 10.1152/JAPPLPHYSIOL.00389.2006
[7]  
Fraser J. R. E., 1996, EXTRACELLULAR MATRIX, V1, P110
[8]   COLLAGEN TYPE-II DIFFERS FROM TYPE-I IN NATIVE MOLECULAR PACKING [J].
GRYNPAS, MD ;
EYRE, DR ;
KIRSCHNER, DA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 626 (02) :346-355
[9]   INTERMOLECULAR SPACE OF RECONSTITUTED COLLAGEN FIBRILS [J].
KATZ, EP ;
LI, ST .
JOURNAL OF MOLECULAR BIOLOGY, 1973, 73 (03) :351-369
[10]   STRUCTURE AND FUNCTION OF BONE COLLAGEN FIBRILS [J].
KATZ, EP ;
LI, S .
JOURNAL OF MOLECULAR BIOLOGY, 1973, 80 (01) :1-15