Changes in properties of white shrimp (Litopenaeus vannamei) protein during thermal denaturation

被引:23
作者
Gao, Ruichang [2 ]
Feng, Xueping [2 ]
Li, Wenwen [2 ]
Yuan, Li [2 ]
Ge, Jing [2 ]
Lu, Daoli [2 ]
Chen, Bin [2 ]
Yu, Gang [1 ]
机构
[1] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
[2] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
关键词
Raman spectroscopy; white shrimp; protein; structures; denaturation; WATER-HOLDING CAPACITY; MERLUCCIUS L. MUSCLE; STRUCTURAL-CHANGES; RAMAN-SPECTROSCOPY; QUALITY; MEAT; GELS;
D O I
10.1007/s10068-016-0003-0
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Changes in white shrimp (Litopenaeus vannamei) protein during thermal denaturation were studied using Raman spectroscopy and isotopic H/D exchange. Denaturation of shrimp protein began after heating for 10 min at 50A degrees C. A decrease in the percentage of alpha-helices accompanied by an increase in the percentage of beta-sheets occurred while the total percentage of disordered structures increased. With extension of the exchange time, the relative intensity of the O-D bond increased, accompanied by a higher relative O-D bond intensity for heated shrimp, compared with unheated shrimp. H/D exchange revealed a higher rate of deuteration kinetics in heated shrimp than for unheated shrimp, especially during the first 2 h, consistent with water loss from denatured white shrimp protein. Physical property changes in muscle tissue can be caused by changes in hydrogen bonding and hydrophobicity during thermal processes.
引用
收藏
页码:21 / 26
页数:6
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