Periplocin Extracted from Cortex Periplocae Induced Apoptosis of Gastric Cancer Cells via the ERK1/2-EGR1 Pathway

被引：49
作者：
Li, Lei [1 ]
Zhao, Lian-Mei [1 ]
Dai, Su-li [1 ]
Cui, Wen-Xuan [1 ]
Lv, Hui-Lai [1 ]
Chen, Liang [1 ]
Shan, Bao-En [1 ]
机构：
[1] Hebei Med Univ, Hosp 4, Res Ctr, 12 Jiankang Rd, Shijiazhuang 050011, Peoples R China
来源：
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2016年 / 38卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Periplocin; Apoptosis; Gastric cancer cell; EGR1; GROWTH-FACTOR RECEPTOR; IN-VITRO; PROLIFERATION; EXPRESSION; SURVIVIN; EGR-1; BETA; VIVO;
D O I：
10.1159/000445555
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background/Aims: Periplocin is extracted from the traditional herbal medicine cortex periplocae, which has been reported to suppress the growth of cancer cells. However, little is known about its effect on gastric cancer cells. Methods: Gastric cancer cells were treated with periplocin, and cell viability was assessed using MIS assay. Flow cytometry and TUNEL staining were performed to evaluate apoptosis, and protein expression was examined by western blotting. Microarray analysis was used to screen for changes in related genes. Results: We found that periplocin had an inhibitory effect on gastric cancer cell viability in a dose-dependent manner. Periplocin inhibited cell viability via the ERK1/2-EGR1 pathway to induce apoptosis. Periplocin also inhibited the growth of tumor xenografts and induced apoptosis in vivo. Conclusion: Our results show that periplocin inhibits the proliferation of gastric cancer cells and induces apoptosis in vitro and in vivo, indicating its potential to be used as an antitumor drug. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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页码：1939 / 1951
页数：13
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