High-level expression and characterization of Aspergillus niger ATCC 1015 xylanase B in Komagataella phaffii

被引：14

作者：

Liu, Taiyu ^{[1
]}

Zhang, Jianguo ^{[1
]}

机构：

[1] Univ Shanghai Sci & Technol, Inst Food Sci & Engn, 516 Jungong Rd, Shanghai 200093, Peoples R China

来源：

APPLIED BIOLOGICAL CHEMISTRY | 2018年 / 61卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Xylanase; Aspergillus niger; Food safety; Gene expression; Komagataella phaffii; PICHIA-PASTORIS; INDUSTRIAL APPLICATIONS; EXTRACTION; CLONING; GENE; ENDO-BETA-1,4-XYLANASE; ENZYMES; SAFETY; PLANTS; FOOD;

D O I：

10.1007/s13765-018-0368-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Owing to the safety issues in food and feed industry, the GH11 xylanase B gene from Aspergillus niger ATCC 1015 was cloned and expressed in Komagataella phaffii. The highest xylanase B activity of 1827.19 U/ml was obtained after optimization of temperature, pH, and methanol addition through flask cultivation. The optimal temperature and pH were 55 A degrees C and 5.0, respectively, and the highest relative activity of xylanase B reached 133.20% with the addition of 10 mmol/l cupric ions. Thus, the high-level recombinant xylanase B obtained in this study could have potential applications in food and feed industry.

引用

页码：373 / 381

页数：9

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