Immobilization of glucose oxidase on Fe3O4/SiO2 magnetic nanoparticles

被引：51

作者：

Huang, Jun ^{[1
]}

Zhao, Rong ^{[1
]}

Wang, Hai ^{[1
]}

Zhao, Wenqi ^{[1
]}

Ding, Liyun ^{[1
]}

机构：

[1] Wuhan Univ Technol, Key Lab Fiber Opt Sensing Technol & Informat Proc, Minist Educ, Wuhan 430070, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Activity; Fe3O4/SiO2 magnetic nanoparticles; Glucose oxidase; Immobilization; Stability;

D O I：

10.1007/s10529-010-0217-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucose oxidase (GOD) was covalently immobilized onto Fe3O4/SiO2 magnetic nanoparticles (FSMNs) using glutaraldehyde (GA). Optimal immobilization was at pH 6 with 3-aminopropyltriethoxysilane at 2% (v/v), GA at 3% (v/v) and 0.143 g GOD per g carrier. The activity of immobilized GOD was 4,570 U/g at pH 7 and 50A degrees C. The immobilized GOD retained 80% of its initial activity after 6 h at 45A degrees C while free enzyme retained only 20% activity. The immobilized GOD maintained 60% of its initial activity after 6 cycles of repeated use and retained 75% of its initial activity after 1 month at 4A degrees C whereas free enzymes retained 62% of its activity.

引用

页码：817 / 821

页数：5

共 14 条

[1] Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme [J].

Ahmad, A ;

Akhtar, MS ;

Bhakuni, V .

BIOCHEMISTRY, 2001, 40 (07) :1945-1955

[2] The Preparation and Enzyme Immobilization of Hydrophobic Polysiloxane Supports [J].

Cao, Shunsheng ;

Liu, Bailing .

MACROMOLECULAR BIOSCIENCE, 2009, 9 (04) :361-368

[3] Controlled fabrication of gold nanoparticles biomediated by glucose oxidase immobilized on chitosan layer-by-layer films [J].

Caseli, Luciano ;

dos Santos, David S., Jr. ;

Aroca, Ricardo F. ;

Oliveira, Osvaldo N., Jr. .

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2009, 29 (05) :1687-1690

[4] Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization [J].

Hou, Xiaohui ;

Liu, Bailing ;

Deng, Xiaobo ;

Zhang, Baotan ;

Chen, Hualin ;

Luo, Rong .

ANALYTICAL BIOCHEMISTRY, 2007, 368 (01) :100-110

[5] A new approach for quantitative determination of glucose by using CdSe/ZnS quantum dots [J].

Huang, Chin-Ping ;

Liu, Shu-Wei ;

Chen, Teng-Ming ;

Li, Yaw-Kuen .

SENSORS AND ACTUATORS B-CHEMICAL, 2008, 130 (01) :338-342

[6] Silica-based hybrid materials as biocompatible coatings for glucose sensors [J].

Kros, A ;

Gerritsen, M ;

Sprakel, VSI ;

Sommerdijk, NAJM ;

Jansen, JA ;

Nolte, RJM .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 81 (01) :68-75

[7]

Li QW, 2000, MAT SCI ENG C-BIO S, V11, P67

[8] PREPARATION OF AQUEOUS MAGNETIC LIQUIDS IN ALKALINE AND ACIDIC MEDIA [J].

MASSART, R .

IEEE TRANSACTIONS ON MAGNETICS, 1981, 17 (02) :1247-1248

[9] Improvement of enzyme activity, stability and selectivity via immobilization techniques [J].

Mateo, Cesar ;

Palomo, Jose M. ;

Fernandez-Lorente, Gloria ;

Guisan, Jose M. ;

Fernandez-Lafuente, Roberto .

ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (06) :1451-1463

[10] Ferrocene-modified Fe3O4@SiO2 magnetic nanoparticles as building blocks for construction of reagentless enzyme-based biosensors [J].

Qiu, Jianding ;

Peng, Huaping ;

Liang, Ruping .

ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (11) :2734-2738

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