Rapid solid-phase extraction method for automated gas chromatographic mass spectrometric determination of nicotine in plasma

被引：15
|
作者：
Davoli, E [1 ]
Stramare, L [1 ]
Fanelli, R [1 ]
Diomede, L [1 ]
Salmona, M [1 ]
机构：
[1] Mario Negri Inst Pharmacol Res, I-20157 Milan, Italy
来源：
JOURNAL OF CHROMATOGRAPHY B | 1998年 / 707卷 / 1-2期
关键词：
nicotine;
D O I：
10.1016/S0378-4347(97)00579-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：312 / 316
页数：5
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