Oleic Acid Esterification in Solvent-Free Medium by Yarrowia lipolytica Lipase Immobilized on Fabric Membranes

被引：6
作者：
Meng, Yong-Hong [3 ,4 ]
Chen, Bi-Qiang [3 ]
Yang, Na [4 ]
Wang, Gui-Li [1 ,2 ]
Li, Ying [1 ,2 ]
Tan, Tian-Wei [3 ]
机构：
[1] China Agr Univ, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China
[2] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China
[3] Beijing Univ Chem Technol, Inst Chem Engn, Dept Biochem Engn, Beijing 100029, Peoples R China
[4] Qinhuangdao Leading Sci & Technol Dev Co Ltd, Qinhuangdao 066004, Hebei, Peoples R China
来源：
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY | 2010年 / 4卷 / 01期
关键词：
Lipase; Immobilized; Esterification; Fabric Membranes; ENZYMATIC-SYNTHESIS; BIODIESEL FUEL; OLEATE; OPTIMIZATION; ESTERS;
D O I：
10.1166/jbmb.2010.1068
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Oleic acid is a major component in fatty acid, a by-product in refining of vegetable oil. In this study, oleic acid was esterified with ethanol using Yarrowia lipolytica lipase immobilized on fabric membranes. Water content and ethanol addition method affected the reaction equilibrium. Esterification degree reached 94.5% under optimal water content and stepwise ethanol addition at 30 C. The optimal condition allowed 82 batches and 30 days with >66.3% and 82.6% conversion, respectively, in 1.41 g oleic acid and 20% immobilized Y. lipolytica lipase. The method described is suitable for large-scale production in view of the lower cost, higher substrate concentration, and greater production volume without use of an organic solvent.
引用
收藏
页码：73 / 78
页数：6
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