Production and application of LPA polyclonal antibody

被引：20

作者：

Chen, JH ^{[1
]}

Zou, F ^{[1
]}

Wang, ND ^{[1
]}

Xie, SW ^{[1
]}

Zhang, X ^{[1
]}

机构：

[1] Peking Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 15期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/S0960-894X(00)00325-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

By using colloidal gold as a hapten carrier, a kind of antibody against lysophosphatidic acid (LPA) was developed and used to successfully detect 500 ng/mL LPA in dot immunogold filtration assay. Such application of the LPA antibody could offer us a way to diagnose ovarian cancer at its early stage. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1691 / 1693

页数：3

共 10 条

[1] A METHOD FOR THE PRODUCTION OF HIGHLY SPECIFIC POLYCLONAL ANTIBODIES [J].

DIANO, M ;

LEBIVIC, A ;

HIRN, M .

ANALYTICAL BIOCHEMISTRY, 1987, 166 (01) :224-229

[2] GROWTH FACTOR-LIKE EFFECTS OF LYSOPHOSPHATIDIC ACID, A NOVEL LIPID MEDIATOR [J].

JALINK, K ;

HORDIJK, PL ;

MOOLENAAR, WH .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 1994, 1198 (2-3) :185-196

[3] LYSOPHOSPHATIDIC ACID, A MULTIFUNCTIONAL PHOSPHOLIPID MESSENGER [J].

MOOLENAAR, WH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (22) :12949-12952

[4]

Moolenaar Wouter H., 1994, Trends in Cell Biology, V4, P213, DOI 10.1016/0962-8924(94)90144-9

[5]

SVENDSEN L, 1995, LAB ANIM SCI, V45, P89

[6]

Tatsumi Noriyuki, 1993, Osaka City Medical Journal, V39, P167

[7]

TIGYI G, 1992, J BIOL CHEM, V267, P21360

[8] LYSOPHOSPHATIDIC ACIDS INDUCE PROLIFERATION OF CULTURED VASCULAR SMOOTH-MUSCLE CELLS FROM RAT AORTA [J].

TOKUMURA, A ;

IIMORI, M ;

NISHIOKA, Y ;

KITAHARA, M ;

SAKASHITA, M ;

TANAKA, S .

AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (01) :C204-C210

[9] Lysophosphatidic acid as a potential biomarker for ovarian and other gynecologic cancers [J].

Xu, Y ;

Shen, ZZ ;

Wiper, DW ;

Wu, MZ ;

Morton, RE ;

Elson, P ;

Kennedy, AW ;

Belinson, J ;

Markman, M ;

Casey, G .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1998, 280 (08) :719-723

[10]

Xu Y, 1995, CLIN CANCER RES, V1, P1223

← 1 →