Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection

被引：14

作者：

Chen, Kuan-Hung ^{[1
,2
]}

Yang, Chien-Hui ^{[1
]}

Cheng, Jiin-Tsuey ^{[2
]}

Wu, Chih-Hsien ^{[1
,2
]}

Sy, Wei-Dih ^{[1
]}

Lin, Chung-Ren ^{[1
,3
]}

机构：

[1] Chang Gung Univ, Kaohsiung Med Ctr, Chang Gung Mem Hosp, Dept Anesthesiol,Coll Med, Kaohsiung, Taiwan

[2] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 80424, Taiwan

[3] Natl Taiwan Univ, Coll Med, Dept Anesthesiol, Taipei 10764, Taiwan

来源：

JOURNAL OF BIOMEDICAL SCIENCE | 2010年 / 17卷

关键词：

NF-KAPPA-B; MESSENGER-RNA; TRANSCRIPTION FACTOR; NEUROPATHIC PAIN; CHROMOSOME; SPINAL-CORD; GENE; CLONING; INJURY; LOCALIZATION;

D O I：

10.1186/1423-0127-17-41

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background: Several molecular changes occur following axotomy, such as gene up-regulation and down-regulation. In our previous study using Affymetrix arrays, it was found that after the axotomy of sciatic nerve, there were many novel genes with significant expression changes. Among them, neuronatin (Nnat) was the one which expression was significantly up-regulated. Nnat was identified as a gene selectively expressed in neonatal brains and markedly reduced in adult brains. The present study investigated whether the expression of Nnat correlates with symptoms of neuropathic pain in adult rats with transected sciatic nerve. Methods: Western blotting, immunohistochemistry, and the Randall and Selitto test were used to study the protein content, and subcellular localization of Nnat in correlation with pain-related animal behavior. Results: It was found that after nerve injury, the expression of Nnat was increased in total protein extracts. Unmyelinated C-fiber and thinly myelinated A-delta fiber in adult dorsal root ganglions (DRGs) were the principal sub-population of primary afferent neurons with distributed Nnat. The increased expression of Nnat and its subcellular localization were related to mechanical hyperalgesia. Conclusions: The results indicated that there was significant correlation between mechanical hyperalgesia in axotomy of sciatic nerve and the increased expression of Nnat in C-fiber and A-delta fiber of adult DRG neurons.

引用

页数：7