Role of glutathione in ethanol stress tolerance in yeast Pachysolen tannophilus

被引：25

作者：

Saharan, Rajesh Kumar ^{[1
]}

Kanwal, Smita ^{[1
]}

Sharma, Sukesh Chander ^{[1
]}

机构：

[1] Panjab Univ, Dept Biochem, Chandigarh 160014, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 397卷 / 02期

关键词：

Oxidative stress tolerance; Pachysolen tannophilus; Glutathione; INDUCED CELL-DEATH; SACCHAROMYCES-CEREVISIAE; OXIDATIVE STRESS; PLASMA-MEMBRANE; ADAPTATION; MECHANISMS; METABOLITE; EXPRESSION; RESISTANCE;

D O I：

10.1016/j.bbrc.2010.05.107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of glutathione enrichment and depletion on the survival of Pachysolen tannophilus after ethanol stress was investigated. In this work, we verified that both control and glutathione deficient yeast cells were much more oxidized after ethanol stress. Depletion of cellular glutathione enhanced the sensitivity to ethanol and suppressed the adaptation. Incubation of the cell with amino acids constituting glutathione (Glu, Cys, Gly) increased the intracellular glutathione content, and subsequently the cell acquired resistance against ethanol. The level of reactive oxygen species, protein carbonyl, and lipid peroxidation in glutathione enriched groups were also studied. These results strongly suggest that intracellular glutathione plays an important role in the adaptive response in P. tannophilus to ethanol induced oxidative stress. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：307 / 310

页数：4

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