Purification of NADH: Hypothiocyanite oxidoreductase in Streptococcus sanguis

被引：15
作者：
Courtois, P
Pourtois, M
机构：
[1] Laboratory of Stomatology, Faculty of Medicine, Free University of Brussels, Brussels
[2] Laboratory of Stomatology, Brussels Free University, B-1070 Brussels
来源：
BIOCHEMICAL AND MOLECULAR MEDICINE | 1996年 / 57卷 / 02期
关键词：
D O I：
10.1006/bmme.1996.0019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NADH: hypothiocyanite oxidoreductase (NHOR) activity, found in some oral Streptococci, is postulated to protect these microorganisms against salivary peroxidase-produced hypothiocyanite, NHOR, however, has not been purified so far. The purification of NHOR from crude extracts of Streptococcus sanguis NCTC 7863 strain (by ultrafiltation and anion-exchange chromatography) revealed one fraction of 125 +/- 1 kDa. However, SDS-PAGE electrophoresis provided a single protein of 21.1 +/- 1.2 kDa, This last discovery suggests that NHOR enzyme is a hexameric complex having six subunits. (C) 1996 Academic Press, Inc.
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页码：134 / 138
页数：5
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