Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

被引：60
作者：
Bareh, Gihan M. [1 ,2 ]
Jacoby, Ethan [2 ]
Binkley, Peter [2 ]
Chang, Tien-cheng Arthur [2 ]
Schenken, Robert S. [2 ]
Robinson, Randal D. [2 ]
机构：
[1] Loma Linda Univ, Loma Linda Univ Hlth Care, Dept Reprod Endocrinol & Infertil, Loma Linda, CA 92350 USA
[2] Univ Texas Hlth Sci Ctr San Antonio, San Antonio, TX 78229 USA
来源：
FERTILITY AND STERILITY | 2016年 / 105卷 / 02期
关键词：
Sperm DNA; recurrent pregnancy loss; unexplained infertility; TUNEL assay; CHROMATIN-STRUCTURE ASSAY; DNA FRAGMENTATION; EMBRYO DEVELOPMENT; HUMAN SPERMATOZOA; HUMAN-FERTILITY; DAMAGE; INTEGRITY; MISCARRIAGE; ABORTION; FERTILIZATION;
D O I：
10.1016/j.fertnstert.2015.10.033
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL). Design: Prospective, cohort study. Setting: Academic tertiary care center. Patient(s): Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility. Intervention(s): Semen samples were collected by masturbation after 48-72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with > 20 x 10(6) spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis. Main Outcome Measure(s): Sperm DNA fragmentation in both groups. Result(s): Mean DNA fragmentation (mean +/- SD) was significantly more in men with RPL (36.8 +/- 5) compared with controls (9.4 +/- 2.7). Conclusion(s): Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters. (C) 2016 by American Society for Reproductive Medicine.
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页码：329 / +
页数：9
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