Optimization of a miniaturized solid-phase microextraction method followed by gas chromatography mass spectrometry for the determination of twenty four volatile and semivolatile compounds in honey from Galicia (NW Spain) and foreign countries

被引：15

作者：

Vazquez, Lua ^{[1
]}

Celeiro, Maria ^{[1
]}

Sergazina, Meruyert ^{[1
,2
]}

Dagnac, Thierry ^{[3
]}

Llompart, Maria ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Dept Analyt Chem Nutr & Food Sci, CRETUS Inst, E-15782 Santiago De Compostela, Spain

[2] Abai Kazakh Natl Pedag Univ, Inst Nat Sci & Geog, Dept Chem, Alma Ata, Kazakhstan

[3] Galician Agcy Food Qual, Agron Res Ctr AGACAL CIAM, Unit Organ Contaminants, Apartado 10, E-15080 La Coruna, Spain

来源：

SUSTAINABLE CHEMISTRY AND PHARMACY | 2021年 / 21卷

关键词：

Honey; Miniaturized solid phase microextraction; Experimental design; GC-MS; BOTANICAL ORIGIN; EXTRACTION; PROFILES; MARKERS;

D O I：

10.1016/j.scp.2021.100451

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An analytical miniaturized methodology based on solid-phase-microextraction (mini_SPME) followed by gas chromatography coupled to mass spectrometry (GC-MS) has been developed for the identification of volatile and semivolatile compounds in honey samples. The main influential experimental parameters, such as the type of fibre coating, extraction temperature, solvent addition, extraction mode, ionic strength, and sample dilution were optimized. A design of experiments (DOE) was conducted including twenty-four target compounds. The final extraction conditions comprised the use of 200 mg of honey mixed with 200 mu L of water (100%, w/v), employing a DVB/CAR/PDMS fibre in the headspace mode at 100 degrees C for 30 min. The mini_SPME-GC-MS method was successfully validated in terms of linearity, repeatability, reproducibility and accuracy. Finally, it was applied to a broad range of varieties of real honey samples from Galicia (NW Spain), as well as some foreign honeys, demonstrating suitability.

引用

页数：10

共 31 条

[1] Aroma investigation of unifloral Greek citrus honey using solid-phase microextraction coupled to gas chromatographic-mass spectrometric analysis [J].