Isothermal Detection of DNA by Beacon-Assisted Detection Amplification

被引：139
作者：
Connolly, Ashley R. [1 ]
Trau, Matt [1 ]
机构：
[1] Univ Queensland, Ctr Biomarker Res & Dev, AIBN, Brisbane, Qld 4072, Australia
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 15期
关键词：
biosensors; DNA detection; DNA nanomachines; molecular devices; nanobiotechnology; STRAND DISPLACEMENT AMPLIFICATION; NUCLEIC-ACIDS; POLYMERASE; REPLICATION; INVITRO;
D O I：
10.1002/anie.200906992
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Not bad at all: A simple biological circuit composed of two integrated molecular switches was developed for the detection and amplification of a specific nucleic acid in a biological extract. The nucleic acid is recognized by a molecular beacon, then a DNA polymerase and nicking endonuclease are used to rapidly amplify the signal for sensitive detection of the nucleic acid. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2720 / 2723
页数：4
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