Endotoxic and immunobiological activities of a chemically synthesized lipid A of Helicobacter pylori strain 206-1

被引：51

作者：

Ogawa, T

Asai, Y

Sakai, Y

Oikawa, M

Fukase, K

Suda, Y

Kusumoto, S

Tamura, T

机构：

[1] Department of Oral Microbiology, Asahi University School of Dentistry, Motosu-gun, Gifu 501-0296, 1851-1 Hozumi, Hozumi-cho

[2] Nutrition Research Institute, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601

[3] Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043

[4] Department of Bioengineering, Faculty of Engineering, Kagoshima University, Kagoshima 890-0065

[5] Department of Bacteriology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8131

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2003年 / 36卷 / 1-2期

基金：

日本学术振兴会;

关键词：

synthetic lipid A; cell activation; toll-like receptor; Helicobacter pylori;

D O I：

10.1016/S0928-8244(03)00093-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A synthetic lipid A of Helicobacter pylori strain 206-1 (compound HP206-1), which is similar to its natural lipid A, exhibited no or very low endotoxic activities as compared to Escherichia coli-type synthetic lipid A (compound 506), Furthermore, compound HP206-1 as well as its natural lipid A demonstrated no or very low mitogenic responses in murine spleen cell. On the other hand, compound HP206-1 showed a weaker but significant production of interleukin-8 in a gastric cancer cell line, MKN-1, in comparison with compound 506. Furthermore, compound HP206-1 exhibited induction of tumor necrosis factor-a production in human peripheral blood mononuclear cells and the cytokine production was clearly inhibited by mouse anti-human Toll-like receptor (TLR) 4 monoclonal antibody HTA125. Our findings indicate that the chemically synthesized lipid A, mimicking the natural lipid A portion of lipopolysaccharide from H. pylori strain 206-1, has a low endotoxic potency and immunobiological activities, and is recognized by TLR4. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：1 / 7

页数：7

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