Modern Approaches in the Identification and Quantification of Immunogenic Peptides in Cereals by LC-MS/MS

被引:36
作者
Alves, Thais O. [1 ,2 ]
D'Almeida, Carolina T. S. [1 ,2 ]
Scherf, Katharina A. [3 ]
Ferreira, Mariana S. L. [1 ,2 ]
机构
[1] Fed Univ State Rio De Janeiro UNIRIO, Lab Bioact, Food & Nutr Grad Program PPGAN, Rio De Janeiro, Brazil
[2] Univ Fed Estado Rio de Janeiro, Lab Prot Biochem, Ctr Innovat Mass Spectrometry LBP IMasS, Rio de Janeiro, Brazil
[3] Karlsruhe Inst Technol, Inst Appl Biosci, Dept Bioact & Funct Food Chem, Karlsruhe, Germany
来源
FRONTIERS IN PLANT SCIENCE | 2019年 / 10卷
关键词
allergenic peptides; cereals; gluten; LC coupled to mass spectrometry; multiple reaction monitoring; prolamins; proteomics; MASS-SPECTROMETRY; CELIAC-DISEASE; WHEAT FLOURS; BARLEY CONTAMINATION; TARGETED PROTEOMICS; GLUTEN PEPTIDES; PROTEINS; GRAIN; QUANTITATION; EPITOPES;
D O I
10.3389/fpls.2019.01470
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Celiac disease (CD) is an immunogenic disorder that affects the small intestine. It is caused by the ingestion of gluten, a protein network formed by prolamins and glutelins from cereals such as wheat, barley, rye and, possibly, oats. For predisposed people, gluten presents epitopes able to stimulate T-cells causing symptoms like nausea, vomiting, diarrhea, among others unrelated to the gastrointestinal system. The only treatment for CD is to maintain a gluten-free diet, not exceeding 20 mg/kg of gluten, what is generally considered the safe amount for celiacs. Due to this context, it is very important to identify and quantify the gluten content of food products. ELISA is the most commonly used method to detect gluten traces in food. However, by detecting only prolamins, the results of ELISA tests may be underestimated. For this reason, more reliable and sensitive assays are needed to improve gluten quantification. Because of high sensitivity and the ability to detect even trace amounts of peptides in complex matrices, the most promising approaches to verify the presence of gluten peptides in food are non-immunological techniques, like liquid chromatography coupled to mass spectrometry. Different methodologies using this approach have been developed and described in the last years, ranging from non-targeted and exploratory analysis to targeted and specific methods depending on the purpose of interest. Non-targeted analyses aim to define the proteomic profile of the sample, while targeted analyses allow the search for specific peptides, making it possible to quantify them. This review aims to gather and summarize the main proteomic techniques used in the identification and quantitation of gluten peptides related to CD-activity and gluten-related allergies.
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页数:13
相关论文
共 88 条
[1]   Evaluation of Qualitative and Quantitative Immunoassays To Detect Barley Contamination in Gluten-Free Beer with Confirmation Using LC/MS/MS [J].
Allred, Laura K. ;
Voyksner, Jennifer A. Sealey ;
Voyksner, Robert D. .
JOURNAL OF AOAC INTERNATIONAL, 2014, 97 (06) :1615-1625
[2]   Integration of transcriptomic and proteomic data from a single wheat cultivar provides new tools for understanding the roles of individual alpha gliadin proteins in flour quality and celiac disease [J].
Altenbach, Susan B. ;
Vensel, William H. ;
DuPont, Frances M. .
JOURNAL OF CEREAL SCIENCE, 2010, 52 (02) :143-151
[3]  
Alves T.O., 2017, Determination of Gluten Peptides Associated with Celiac Disease by Mass Spectrometry, DOI DOI 10.5772/67547
[4]   Immunogenic and allergenic profile of wheat flours from different technological qualities revealed by ion mobility mass spectrometry [J].
Alves, Thais O. ;
D'Almeida, Carolina T. S. ;
Victorio, Veronica C. M. ;
Souza, Gustavo H. M. F. ;
Cameron, L. C. ;
Ferreira, Mariana S. L. .
JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2018, 73 :67-75
[5]   Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins [J].
Anderson, L ;
Hunter, CL .
MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (04) :573-588
[6]  
[Anonymous], 2008, Codex stan, P118
[7]   Mass Spectrometry, Review of the Basics: Ionization [J].
Awad, Hanan ;
Khamis, Mona M. ;
El-Aneed, Anas .
APPLIED SPECTROSCOPY REVIEWS, 2015, 50 (02) :158-175
[8]  
Banerjee B, 2010, NUTR MANAGEMENT DIGE, DOI [10.1201/9781420086553, DOI 10.1201/9781420086553]
[9]   UniProt: the universal protein knowledgebase [J].
Bateman, Alex ;
Martin, Maria Jesus ;
O'Donovan, Claire ;
Magrane, Michele ;
Alpi, Emanuele ;
Antunes, Ricardo ;
Bely, Benoit ;
Bingley, Mark ;
Bonilla, Carlos ;
Britto, Ramona ;
Bursteinas, Borisas ;
Bye-A-Jee, Hema ;
Cowley, Andrew ;
Da Silva, Alan ;
De Giorgi, Maurizio ;
Dogan, Tunca ;
Fazzini, Francesco ;
Castro, Leyla Garcia ;
Figueira, Luis ;
Garmiri, Penelope ;
Georghiou, George ;
Gonzalez, Daniel ;
Hatton-Ellis, Emma ;
Li, Weizhong ;
Liu, Wudong ;
Lopez, Rodrigo ;
Luo, Jie ;
Lussi, Yvonne ;
MacDougall, Alistair ;
Nightingale, Andrew ;
Palka, Barbara ;
Pichler, Klemens ;
Poggioli, Diego ;
Pundir, Sangya ;
Pureza, Luis ;
Qi, Guoying ;
Rosanoff, Steven ;
Saidi, Rabie ;
Sawford, Tony ;
Shypitsyna, Aleksandra ;
Speretta, Elena ;
Turner, Edward ;
Tyagi, Nidhi ;
Volynkin, Vladimir ;
Wardell, Tony ;
Warner, Kate ;
Watkins, Xavier ;
Zaru, Rossana ;
Zellner, Hermann ;
Xenarios, Ioannis .
NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D158-D169
[10]  
Belitz H.-D., 2009, Food Chemistry, V4th, P374