Gold nanoparticles functionalized with Ru(II)bipyridyl labeled DNA as a luminescent probe for the sensitive determination of DNase I

被引：7
作者：
Cao, Xiu-Hui [1 ]
Wang, Qiong [1 ]
Li, Jing [1 ]
Yi, Changqing [2 ,3 ]
Li, Mei-Jin [1 ]
机构：
[1] Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety Fujian, Key Lab Analyt Sci Food Safety & Biol, Minist Educ,Dept Chem, Fuzhou 350116, Fujian, Peoples R China
[2] Sun Yat Sen Univ, Sch Engn, Key Lab Sensing Technol & Biomed Instruments Guan, Guangzhou 510006, Guangdong, Peoples R China
[3] Sun Yat Sen Univ Shenzhen, Res Inst, Shenzhen, Peoples R China
来源：
MICROCHIMICA ACTA | 2017年 / 184卷 / 09期
关键词：
Gold nano probes; Ru(II) complex; Enzyme activity assay; Deoxyribonuclease I; DEOXYRIBONUCLEASE-I; BINDING; ASSAY; COMPLEXES; FLUORESCENCE; PLATFORM; POLYMER;
D O I：
10.1007/s00604-017-2330-0
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The authors describe a fluorometric method for the determination of the activity of the enzyme DNase I. Gold nanoparticles were functionalized with a Ru(II) bipyridyl complex to which dsDNA chains were covalatently linked via carboxy groups. The emission of the Ru(II) complex (measured at excitation/emission wavlengths of 450/629 nm) is quenched by the gold nanoparticles due to surface energy transfer. The emission is restored as soon as the Ru(II)-labeled dsDNA is cut off by the action of DNase I. Based on this mechanism, the DNase I activity in vitro was detected with high sensitivity. The effect of the different lengths of DNA chains on luminescence quenching and sensing ability was studied. The intensity of restored luminescence is linearly related to the activity of DNase I in the range from 4 to 400 mU.mL(-1) with a detection limit of 50 mu U.mL(-1).
引用
收藏
页码：3273 / 3279
页数：7
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