Proteomic Characterization of High-Density Lipoprotein Particles from Non-Diabetic Hemodialysis Patients

被引:14
作者
Florens, Nans [1 ,2 ]
Calzada, Catherine [1 ]
Delolme, Frederic [3 ]
Page, Adeline [3 ]
Guebre Egziabher, Fitsum [1 ,2 ]
Juillard, Laurent [1 ,2 ]
Soulage, Christophe O. [1 ]
机构
[1] Univ Claude Bernard Lyon 1, Univ Lyon, INSA Lyon, CarMeN,INSERM,U1060,U1397,INRA, F-69621 Villeurbanne, France
[2] Hosp Civils Lyon, Hop E Herriot, Serv Nephrol Hypertens Hemodialyse, F-69003 Lyon, France
[3] Univ Claude Bernard Lyon 1, ENS Lyon, Prot Sci Facil, SFR BioSci,CNRS,UMS3444,Inserm,US8, F-69007 Lyon, France
关键词
HDL cholesterol; lipoproteins; cardiovascular risk; proteomic; mass spectrometry; hemodialysis; CARDIOVASCULAR EVENTS; HDL COMPOSITION; KIDNEY-DISEASE; MODALITIES; PROTEINS;
D O I
10.3390/toxins11110671
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Chronic kidney disease is associated with an increased cardiovascular risk, and altered biological properties of high-density lipoproteins (HDL) may play a role in these events. This study aimed to describe the HDL proteome from non-diabetic hemodialysis patients and identify potential pathways affected by the dysregulated expression of HDL proteins. HDL were sampled from nine non-diabetic hemodialysis (HD) and eight control patients. Samples were analyzed using a nano-RSLC coupled with a Q-Orbitrap. Data were processed by database searching using SequestHT against a human Swissprot database and quantified with a label-free quantification approach. Proteins that were in at least five of the eight control and six of the nine HD patients were analyzed. Analysis was based on pairwise ratios and the ANOVA hypothesis test. Among 522 potential proteins, 326 proteins were identified to be in the HDL proteome from HD and control patients, among which 10 were significantly upregulated and nine downregulated in HD patients compared to the control patients (p < 0.05). Up and downregulated proteins were involved in lipid metabolism, hemostasis, wound healing, oxidative stress, and apoptosis pathways. This difference in composition could partly explain HDL dysfunction in the chronic kidney disease (CKD) population and participate in the higher cardiovascular risk observed in this population.
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页数:10
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共 18 条
[11]   Proteome profiles of HDL particles of patients with chronic heart failure are associated with immune response and also include bacteria proteins [J].
Oberbach, Andreas ;
Adams, Volker ;
Schlichting, Nadine ;
Heinrich, Marco ;
Kullnick, Yvonne ;
Lehmann, Stefanie ;
Lehmann, Sven ;
Feder, Stefan ;
Correia, Joao Carlos ;
Mohr, Friedrich-Wilhelm ;
Voelker, Uwe ;
Jehmlich, Nico .
CLINICA CHIMICA ACTA, 2016, 453 :114-122
[12]   Quantifying HDL proteins by mass spectrometry: how many proteins are there and what are their functions? [J].
Shao, Baohai ;
Heinecke, Jay W. .
EXPERT REVIEW OF PROTEOMICS, 2018, 15 (01) :31-40
[13]   A Cluster of Proteins Implicated in Kidney Disease Is Increased in High-Density Lipoprotein Isolated from Hemodialysis Subjects [J].
Shao, Baohai ;
de Boer, Ian ;
Tang, Chongren ;
Mayer, Philip S. ;
Zelnick, Leila ;
Afkarian, Maryam ;
Heinecke, Jay W. ;
Himmelfarb, Jonathan .
JOURNAL OF PROTEOME RESEARCH, 2015, 14 (07) :2792-2806
[14]   HDL in Children with CKD Promotes Endothelial Dysfunction and an Abnormal Vascular Phenotype [J].
Shroff, Rukshana ;
Speer, Thimoteus ;
Colin, Sophie ;
Charakida, Marietta ;
Zewinger, Stephen ;
Staels, Bart ;
Chinetti-Gbaguidi, Giulia ;
Hettrich, Inga ;
Rohrer, Lucia ;
O'Neill, Francis ;
McLoughlin, Eve ;
Long, David ;
Shanahan, Catherine M. ;
Landmesser, Ulf ;
Fliser, Danilo ;
Deanfield, John E. .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2014, 25 (11) :2658-2668
[15]   Plasma Beta-Trace Protein as a Marker of Residual Renal Function: The Effect of Different Hemodialysis Modalities and Intra-Individual Variability over Time [J].
Van Craenenbroeck, Amaryllis H. ;
Bragfors-Helin, Ann-Christin ;
Qureshi, Abdul Rashid ;
Lindholm, Bengt ;
Sjoberg, Bodil ;
Anderstam, Bjorn ;
Heimburger, Olof ;
Stenvinkel, Peter ;
Barany, Peter .
KIDNEY & BLOOD PRESSURE RESEARCH, 2017, 42 (05) :877-885
[16]   Causes of Dysregulation of Lipid Metabolism in Chronic Renal Failure [J].
Vaziri, Nosratola D. .
SEMINARS IN DIALYSIS, 2009, 22 (06) :644-651
[17]   Atorvastatin in patients with type 2 diabetes mellitus undergoing hemodialysis [J].
Wanner, C ;
Krane, V ;
März, W ;
Olschewski, M ;
Mann, JFE ;
Ruf, G ;
Ritz, E .
NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (03) :238-248
[18]   Dysfunctional High-Density Lipoprotein in Patients on Chronic Hemodialysis [J].
Yamamoto, Suguru ;
Yancey, Patricia G. ;
Ikizler, Alp ;
Jerome, W. Gray ;
Kaseda, Ryohei ;
Cox, Brian ;
Bian, Aihua ;
Shintani, Ayumi ;
Fogo, Agnes B. ;
Linton, MacRae F. ;
Fazio, Sergio ;
Kon, Valentina .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2012, 60 (23) :2372-2379