Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

被引：112

作者：

McQuade, Lindsey E. ^{[1
]}

Ma, Jie ^{[2
]}

Lowe, Graeme ^{[2
]}

Ghatpande, Ambarish ^{[2
]}

Gelperin, Alan ^{[2
]}

Lippard, Stephen J. ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

[2] Monell Chem Senses Ctr, Philadelphia, PA 19104 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 19期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

fluorescent sensing; NO; olfaction; trappable probe; fluorescence microscopy; LONG-TERM POTENTIATION; VOMERONASAL SYSTEM; SYNTHASE; HETEROGENEITY; INHIBITION; EXPRESSION; SURVIVAL; BIOLOGY; BRAIN; MICE;

D O I：

10.1073/pnas.0914794107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu(2)(FL2E) and the membrane-impermeable acid derivative Cu(2)(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu(2)(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.

引用

页码：8525 / 8530

页数：6

共 37 条

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