Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino)

被引：89

作者：

Zhu, Bei-Wei ^{[1
]}

Zhou, Da-yong ^{[1
]}

Li, Tao ^{[1
]}

Yan, Shuo ^{[2
]}

Yang, Jing-feng ^{[1
]}

Li, Dong-mei ^{[1
]}

Dong, Xiu-ping ^{[1
]}

Murata, Yoshiyuki ^{[3
]}

机构：

[1] Dalian Polytech Univ, Coll Biol & Food Engn, Dalian 116034, Peoples R China

[2] Univ Guelph, Ontario Agr Coll, Dept Food Sci, Guelph, ON N1G 2W1, Canada

[3] Okayama Univ, Fac Agr, Dept Biol Resources Chem, Okayama 7008530, Japan

来源：

FOOD CHEMISTRY | 2010年 / 121卷 / 03期

基金：

国家高技术研究发展计划(863计划);

关键词：

Haliotis Discus Hannai Ino; Gonad; Sulphated Polysaccharide; Chemical composition; Free radical scavenging activity; ANTIOXIDANT ACTIVITIES; LAMINARIA-JAPONICA; ALKOXY RADICALS; GREEN TEA; FRACTIONS; VITRO; DERIVATIVES; SINENSIS;

D O I：

10.1016/j.foodchem.2010.01.010

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PW(XL) column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE). (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：712 / 718

页数：7

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