Chemical composition and free radical scavenging activities of a sulphated polysaccharide extracted from abalone gonad (Haliotis Discus Hannai Ino)

被引：88
作者：
Zhu, Bei-Wei [1 ]
Zhou, Da-yong [1 ]
Li, Tao [1 ]
Yan, Shuo [2 ]
Yang, Jing-feng [1 ]
Li, Dong-mei [1 ]
Dong, Xiu-ping [1 ]
Murata, Yoshiyuki [3 ]
机构：
[1] Dalian Polytech Univ, Coll Biol & Food Engn, Dalian 116034, Peoples R China
[2] Univ Guelph, Ontario Agr Coll, Dept Food Sci, Guelph, ON N1G 2W1, Canada
[3] Okayama Univ, Fac Agr, Dept Biol Resources Chem, Okayama 7008530, Japan
来源：
FOOD CHEMISTRY | 2010年 / 121卷 / 03期
基金：
国家高技术研究发展计划(863计划);
关键词：
Haliotis Discus Hannai Ino; Gonad; Sulphated Polysaccharide; Chemical composition; Free radical scavenging activity; ANTIOXIDANT ACTIVITIES; LAMINARIA-JAPONICA; ALKOXY RADICALS; GREEN TEA; FRACTIONS; VITRO; DERIVATIVES; SINENSIS;
D O I：
10.1016/j.foodchem.2010.01.010
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Crude polysaccharides (CPs) were prepared from Haliotis Discus Hannai Ino gonad by protease-hydrolysis combining boiling-methanol extraction. They were purified to homogeneity by gel-filtration chromatography on Sephacryl-S 100 and Sephacryl-S 200, and semi-preparation high performance liquid chromatography (semi-RP-HPLC) equipped with a SOURCE 30Q anion exchange column. The sulphated polysaccharide obtained, named as PAGP, has an average molecular weight (MW) of 12.5 kDa determined by HPLC coupled to a TSK-gel G4000PW(XL) column. Chemical composition analysis indicated it was composed of carbohydrate (50.7%), sulphated group (31.1%), aminohexose (7.1%), aronic acid (6.2%) and protein (2.0%). The carbohydrate part of PAGP was composed of rhamnose (Rha), fucose (Fuc), and galactose (Gal) in molar ratio of 1.0:1.6:2.3. The protein part of PAGP contained 13 kinds of amino acids. Antioxidant assays demonstrated that PAGP possessed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activities than positive controls vitamin C (VC) and vitamin E (VE). (C) 2010 Elsevier Ltd. All rights reserved.
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页码：712 / 718
页数：7
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