Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA

被引：19

作者：

Liu, Jing ^{[1
]}

Yu, Jinshu ^{[1
]}

Wang, Ming ^{[1
]}

Liu, Qun ^{[1
]}

Zhang, Wei ^{[1
]}

Deng, Chong ^{[1
]}

Ding, Jun ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Dept Vet Prevent Med, Parasitol Lab, Beijing, Peoples R China

来源：

VETERINARY PARASITOLOGY | 2007年 / 143卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

Neospora caninum; recombinant tNcSRS2; ELISA; serodiagnosis;

D O I：

10.1016/j.vetpar.2006.08.034

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The truncated NcSRS2 gene (tNcSRS2) by removal of the N-terminal hydrophobic sequence was cloned into the pGEX-6p-1 plasmid and subsequently expressed as a glutathione-S-transferase (GST) fusion protein. The purified recombinant tNcSRS2 protein was specific to Neospora caninum and was used in an ELISA for the diagnosis of neosporosis. There was a good agreement between tNctSRS2-based ELISA and three commercially available diagnostic kits (IDEXX ELISA, HIPRA ELISA and VMRD IFAT). Three hundred dairy cattle serum samples from 9 regions were tested by our ELISA. The herd prevalence was 100% and the overall prevalence was 20.3%. There was a statistically significant difference in seroprevalence between regions (P < 0.01) and an association between abortion history and N. caninum seropositivity. Our study showed that neosporosis in dairy cattle is widespread in China. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：358 / 363

页数：6

共 22 条

[1]

Ahn Hye-Jin, 2003, Korean Journal of Parasitology, V41, P175, DOI 10.3347/kjp.2003.41.3.175

[2] Immune responses during pregnancy in heifers naturally infected with Neospora caninum with and without immunization [J].

Andrianarivo, AG ;

Anderson, ML ;

Rowe, JD ;

Gardner, IA ;

Reynolds, JP ;

Choromanski, L ;

Conrad, PA .

PARASITOLOGY RESEARCH, 2005, 96 (01) :24-31

[3] Serological diagnosis of Neospora caninum infection [J].

Björkman, C ;

Uggla, A .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1999, 29 (10) :1497-1507

[4]

Dubey John P., 2003, Korean Journal of Parasitology, V41, P1, DOI 10.3347/kjp.2003.41.1.1

[5]

DUBEY JP, 1988, J AM VET MED ASSOC, V193, P1259

[6] Serological survey of Neospora caninum infection in dairy cattle herds in Aguascalientes, Mexico [J].

García-Vázquez, Z ;

Cruz-Vázquez, C ;

Medina-Espinoza, L ;

García-Tapia, D ;

Chavarria-Martinez, B .

VETERINARY PARASITOLOGY, 2002, 106 (02) :115-120

[7]

GARCIAVAZQUEZ Z, 2005, VET PARASITOL

[8] Detection of antibodies to Neospora caninum in cattle by enzyme-linked Immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli [J].

Gaturaga, I ;

Chahan, B ;

Xuan, XN ;

Huang, XH ;

Liao, M ;

Fukumoto, S ;

Hirata, H ;

Nishikawa, Y ;

Takashima, Y ;

Suzuki, H ;

Fujisaki, K ;

Sugimoto, C .

JOURNAL OF PARASITOLOGY, 2005, 91 (01) :191-192

[9] Identification of a major surface protein on Neospora caninum tachyzoites [J].

Hemphill, A ;

Gottstein, B .

PARASITOLOGY RESEARCH, 1996, 82 (06) :497-504

[10] Adhesion and invasion of bovine endothelial cells by Neospora caninum [J].

Hemphill, A ;

Gottstein, B ;

Kaufmann, H .

PARASITOLOGY, 1996, 112 :183-197

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