Preparation and cytotoxicity of N-modified chitosan nanoparticles applied in curcumin delivery

被引：64
作者：
Facchi, Suelen P. [1 ]
Scariot, Debora B. [3 ]
Bueno, Pedro V. A. [2 ]
Souza, Paulo R. [2 ]
Figueiredo, Luana C. [1 ]
Follmann, Heveline D. M. [2 ]
Nunes, Catia S. [2 ]
Monteiro, Johny P. [1 ]
Bonafe, Elton G. [1 ]
Nakamura, Celso V. [3 ]
Muniz, Edvani C. [2 ]
Martins, Alessandro F. [1 ,2 ]
机构：
[1] Univ Tecnol Fed Parana UTFPR, Dept Quim, BR-86812120 Apucarana, Brazil
[2] Univ Estadual Maringa, Grp Mat Polimer & Compositos, BR-87020900 Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Dept Ciencias Basicas Saude, BR-87020900 Maringa, Parana, Brazil
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 87卷
关键词：
N; N-Trimethyl chitosan; Nanoparticles; Curcumin; Delivery; Cytotoxicity; ORAL DELIVERY; CHITOSAN/ALGINATE COMPLEXES; POLYELECTROLYTE COMPLEX; BACTERICIDAL ACTIVITY; HYDROGEL BEADS; RELEASE; CELLS; MICROPARTICLES; MICROSPHERES; PERMEABILITY;
D O I：
10.1016/j.ijbiomac.2016.02.063
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nanoparticles (NPs) based on N,N-dimethyl chitosan (DMC) and N,N,N-trimethyl chitosan (TMC), physical crosslinked with sodium tripolyphosphate (TPP) were successful obtained, using water/benzyl alcohol emulsion system. NPs morphologies were evaluated by Scanning Electron Microscopy and Transmission Electron Microscopy. NPs were characterized by Infrared Spectroscopy (FTIR), Thermogravimetric Analysis, Zeta Potential, Differential Scanning Calorimetry and Wide-angle X-ray Scattering. Curcumin (CUR) was loaded onto NPs and controlled release studies were evaluated in simulated intestinal fluid and in simulated gastric fluid. Cytotoxicity assays showed only loaded TMC/TPP particles containing CUR were slightly cytotoxic on human cervical tumor cells (SiHa cells), concerning unloaded TMC/TPP particles. Conversely, loaded NPs (TMC/TPP/CUR and DMC/TPP/CUR), especially TMC/TPP/CUR sample presented greater biocompatibility toward healthy VERO cells than unloaded NPs (TMC/TPP and DMC/TPP). (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：237 / 245
页数：9
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