Direct and reversible immobilization and microcontact printing of functional proteins on glass using a genetically appended silica-binding tag

被引：18

作者：

Coyle, Brandon L. ^{[1
]}

Baneyx, Francois ^{[1
]}

机构：

[1] Univ Washington, Dept Chem Engn, Box 351750, Seattle, WA 98195 USA

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 43期

关键词：

GREEN FLUORESCENT PROTEIN; MOLECULAR BIOMIMETICS; INORGANIC MATERIALS; CLICK CHEMISTRY; PEPTIDE; SURFACE; LITHOGRAPHY; POLYPEPTIDE; GOLD;

D O I：

10.1039/c6cc02660e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fusion of disulfide-constrained or linear versions of the Car9 dodecapeptide to model fluorescent proteins support their on-contact and oriented immobilization onto unmodified glass. Bound proteins can be released and the surface regenerated by incubation with (L)-lysine. This noncovalent chemistry enables rapid and reversibe microcontact printing of tagged proteins and speeds up the production of bicontinuous protein patterns.

引用

页码：7001 / 7004

页数：4

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