Strigolactone mimic 2-nitrodebranone is highly active in Arabidopsis growth and development

被引：11

作者：

Li, Suhua ^{[1
,2
]}

Li, Yuwen ^{[2
]}

Chen, Linhai ^{[3
]}

Zhang, Chi ^{[1
]}

Wang, Fei ^{[2
]}

Li, Haiou ^{[2
,4
]}

Wang, Ming ^{[5
]}

Wang, Yupei ^{[2
]}

Nan, Fajun ^{[3
]}

Xie, Daoxin ^{[2
]}

Yan, Jianbin ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen,Minist Agr, Shenzhen Key Lab Agr Synthet Biol,Genome Anal Lab, Guangdong Lab Lingnan Modern Agr,Shenzhen Branch, Shenzhen 518124, Peoples R China

[2] Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

[4] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China

[5] Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210000, Peoples R China

来源：

PLANT JOURNAL | 2021年 / 107卷 / 01期

基金：

国家重点研发计划;

关键词：

Phytohormone; GR24; 2NOD; Signaling Pathway; Plant growth regulator; F-BOX PROTEIN; STRIGA-HERMONTHICA; GERMINATION STIMULANTS; HORMONE-RECEPTOR; PLANT HORMONES; INHIBITION; RICE; RESPONSES; ACTS; STEREOISOMERS;

D O I：

10.1111/tpj.15274

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Strigolactones play crucial roles in regulating plant architecture and development, as endogenous hormones, and orchestrating symbiotic interactions with fungi and parasitic plants, as components of root exudates. rac-GR24 is currently the most widely used strigolactone analog and serves as a reference compound in investigating the action of strigolactones. In this study, we evaluated a suite of debranones and found that 2-nitrodebranone (2NOD) exhibited higher biological activity than rac-GR24 in various aspects of plant growth and development in Arabidopsis, including hypocotyl elongation inhibition, root hair promotion and senescence acceleration. The enhanced activity of 2NOD in promoting AtD14-SMXL7 and AtD14-MAX2 interactions indicates that the molecular structure of 2NOD is a better match for the ligand perception site pocket of D14. Moreover, 2NOD showed lower activity than rac-GR24 in promoting Orobanche cumana seed germination, suggesting its higher ability to control plant architecture than parasitic interactions. In combination with the improved stability of 2NOD, these results demonstrate that 2NOD is a strigolactone analog that can specifically mimic the activity of strigolactones and that 2NOD exhibits strong potential as a tool for studying the strigolactone signaling pathway in plants.

引用

页码：67 / 76

页数：10

共 46 条

[1] MAX1 encodes a cytochrome P450 family member that acts downstream of MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone [J].