New Ultralow-Cost Telemetric System for a Rapid Electrochemical Detection of Vitamin C in Fresh Orange Juice

被引:35
作者
Barberis, Antonio [2 ]
Bazzu, Gianfranco [1 ]
Calia, Giammario [1 ]
Puggioni, Giulia M. G. [1 ]
Rocchitta, Gaia G. [1 ]
Migheli, Rossana [1 ]
Schirra, Mario [2 ]
Desole, Maria Speranza [1 ]
Serra, Pier Andrea [1 ]
机构
[1] Univ Sassari, Sch Med, Dept Neurosci, I-07100 Sassari, Italy
[2] CNR, Inst Food Sci, I-07040 Sassari, Italy
关键词
L-ASCORBIC-ACID; AMPEROMETRIC DETERMINATION; ELECTRODE; GLUCOSE;
D O I
10.1021/ac100303p
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Ascorbic acid (AA), one of the principal micronutrients in horticultural crops, plays a key role in the human metabolism, and its determination in food products has a great significance. Citrus fruits are rich in AA, but its content is highly susceptible to change during postharvest processing and storage. We present a new ultralow-cost system, constituted of an amperometric microsensor composed of three rod carbon electrodes connected to a telemetric device, for online detection of AA in orange juice, as an alternative to conventional analytical methods. The in vitro calibration, ranged from 0 to 5 mM, and AA juice content was calculated by adding low volumes of sample into an acetate buffer solution at a constant potential of +120 mV vs carbon pseudoreference. This new approach, which is simple, expandable, and inexpensive, seems appropriate for large scale commercial use.
引用
收藏
页码:5134 / 5140
页数:7
相关论文
共 37 条
[1]   A new enzyme electrode based on ascorbate oxidase immobilized in gelatin for specific determination of L-ascorbic acid [J].
Akyilmaz, E ;
Dinçkaya, E .
TALANTA, 1999, 50 (01) :87-93
[2]   Non-spectrophotometric methods for the determination of Vitamin C [J].
Arya, SP ;
Mahajan, M ;
Jain, P .
ANALYTICA CHIMICA ACTA, 2000, 417 (01) :1-14
[3]  
*ASS OFF AN CHEM, 1990, AOAC OFF METH AN ASS, P1058
[4]   Real-Time Monitoring of Brain Tissue Oxygen Using a Miniaturized Biotelemetric Device Implanted in Freely Moving Rats [J].
Bazzu, Gianfranco ;
Puggioni, Giulia G. M. ;
Dedola, Sonia ;
Calia, Giammario ;
Rocchitta, Gaia ;
Migheli, Rossana ;
Desole, Maria S. ;
Lowry, John P. ;
O'Neill, Robert D. ;
Serra, Pier A. .
ANALYTICAL CHEMISTRY, 2009, 81 (06) :2235-2241
[5]   Biotelemetric Monitoring of Brain Neurochemistry in Conscious Rats Using Microsensors and Biosensors [J].
Calia, Giammario ;
Rocchitta, Gaia ;
Migheli, Rossana ;
Puggioni, Giulia ;
Spissu, Ylenia ;
Bazzu, Gianfranco ;
Mazzarello, Vittorio ;
Lowry, John P. ;
O'Neill, Robert D. ;
Desole, Maria S. ;
Serra, Pier A. .
SENSORS, 2009, 9 (04) :2511-2523
[6]   Influence of pencil lead hardness on voltammetric response of graphite reinforcement carbon electrodes [J].
Campos Prado Tavares, Paulo Henrique ;
Sanches Barbeira, Paulo Jorge .
JOURNAL OF APPLIED ELECTROCHEMISTRY, 2008, 38 (06) :827-832
[7]   Electrocatalysis of ascorbic acid ion the glassy carbon electrode chemically modified with polyaniline films [J].
Casella, IG ;
Guascito, MR .
ELECTROANALYSIS, 1997, 9 (18) :1381-1386
[8]   A metabolic switch in brain: glucose and lactate metabolism modulation by ascorbic acid [J].
Castro, Maite A. ;
Beltran, Felipe A. ;
Brauchi, Sebastian ;
Concha, Ilona I. .
JOURNAL OF NEUROCHEMISTRY, 2009, 110 (02) :423-440
[9]   Amperometric Determination of Ascorbic Acid in Real Samples Using a Disposable Screen-Printed Electrode Modified with Electrografted o-Aminophenol Film [J].
Civit, Laia ;
Nassef, Hossam M. ;
Fragoso, Alex ;
O'Sullivan, Ciara K. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (22) :10452-10455
[10]   Meldola's blue immobilized on a SiO2/SnO2/Phosphate xerogel, a new sensor for determination of ascorbic acid in medicine and commercial fruit juice [J].
de Castilho, Rodrigo Ferrari ;
Ribeiro de Souza, Eloana Benassi ;
da Silva Alfaya, Reni Ventura ;
da Silva Alfaya, Antonio Alberto .
ELECTROANALYSIS, 2008, 20 (02) :157-162