New Approaches in Cognitive Neurobiology: Techniques for Two-Photon Vivo Imaging of Cognitively Active Neurons

被引：2

作者：

Roshchina, M. A. ^{[1
]}

Ivashkina, O. I. ^{[1
]}

Anokhin, K. V. ^{[1
,2
,3
]}

机构：

[1] Natl Res Ctr, Kurchatov Inst, Moscow, Russia

[2] Lomonosov Moscow State Univ, Moscow, Russia

[3] Anokhin Inst Normal Physiol, Moscow, Russia

来源：

ZHURNAL VYSSHEI NERVNOI DEYATELNOSTI IMENI I P PAVLOVA | 2017年 / 67卷 / 02期

关键词：

brain; neurons; cognitive activity; learning; memory; two-photon microscopy; optical imaging; fluorescent proteins; genetically-encoded indicators; GECI; GCaMP; c-fos; IN-VIVO; GENE-EXPRESSION; FUNCTIONAL CONNECTOMICS; OLFACTORY-BULB; VISUAL-CORTEX; MOTOR CORTEX; MICROSCOPY; CALCIUM; BRAIN; ARC;

D O I：

10.7868/S0044467717020113

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

This review is focused on the modern methods of in vivo two-photon imaging of activity of neurons involved in cognitive processes in experimental animals. We describe main principles of in vivo two photon microscopy and optical imaging of neuronal activity with two types of calcium sensors: chemical dyes and genetically encoded calcium indicators. We also review a novel approach of in vivo imaging of genomic responses of neocortical neurons to cognitive stimuli using fluorescent reporters for expression of immediate early genes c-fos, Arc and Egr-1 in transgenic mice. We next compare the main advantages and limitations of these approaches and discuss their perspectives for the study of cellular bases of higher brain functions.

引用

收藏

页码：141 / 149

页数：9

相关论文

共 46 条

[31] Learning Enhances Sensory and Multiple Non-sensory Representations in Primary Visual Cortex [J].

Poort, Jasper ;

Khan, Adil G. ;

Pachitariu, Marius ;

Nemri, Abdellatif ;

Orsolic, Ivana ;

Krupic, Julija ;

Bauza, Marius ;

Sahani, Maneesh ;

Keller, Georg B. ;

Mrsic-Flogel, Thomas D. ;

Hofer, Sonja B. .

NEURON, 2015, 86 (06) :1478-1490

[32] Development of Direction Selectivity in Mouse Cortical Neurons [J].

Rochefort, Nathalie L. ;

Narushima, Madoka ;

Grienberger, Christine ;

Marandi, Nima ;

Hill, Daniel N. ;

Konnerth, Arthur .

NEURON, 2011, 71 (03) :425-432

[33] Orchestrated experience-driven Arc responses are disrupted in a mouse model of Alzheimer's disease [J].

Rudinskiy, Nikita ;

Hawkes, Jonathan M. ;

Betensky, Rebecca A. ;

Eguchi, Megumi ;

Yamaguchi, Shun ;

Spires-Jones, Tara L. ;

Hyman, Bradley T. .

NATURE NEUROSCIENCE, 2012, 15 (10) :1422-1429

[34] All-near-infrared multiphoton microscopy interrogates intact tissues at deeper imaging depths than conventional single- and two-photon near-infrared excitation microscopes [J].

Sarder, Pinaki ;

Yazdanfar, Siavash ;

Akers, Walter J. ;

Tang, Rui ;

Sudlow, Gail P. ;

Egbulefu, Christopher ;

Achilefu, Samuel .

JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (10)

[35] In vivo two-photon calcium imaging of neuronal networks [J].

Stosiek, C ;

Garaschuk, O ;

Holthoff, K ;

Konnerth, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (12) :7319-7324

[36] Principles of two-photon excitation microscopy and its applications to neuroscience [J].

Svoboda, Karel ;

Yasuda, Ryohei .

NEURON, 2006, 50 (06) :823-839

[37] Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier [J].

Theer, P ;

Hasan, MT ;

Denk, W .

OPTICS LETTERS, 2003, 28 (12) :1022-1024

[38] Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators [J].

Tian, Lin ;

Hires, S. Andrew ;

Mao, Tianyi ;

Huber, Daniel ;

Chiappe, M. Eugenia ;

Chalasani, Sreekanth H. ;

Petreanu, Leopoldo ;

Akerboom, Jasper ;

McKinney, Sean A. ;

Schreiter, Eric R. ;

Bargmann, Cornelia I. ;

Jayaraman, Vivek ;

Svoboda, Karel ;

Looger, Loren L. .

NATURE METHODS, 2009, 6 (12) :875-U113

[39] Deep two-photon brain imaging with a red-shifted fluorometric Ca2+ indicator [J].

Tischbirek, Carsten ;

Birkner, Antje ;

Jia, Hongbo ;

Sakmann, Bert ;

Konnerth, Arthur .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (36) :11377-11382

[40] CALCIUM CHANNELS, STORES, AND OSCILLATIONS [J].

TSIEN, RW ;

TSIEN, RY .

ANNUAL REVIEW OF CELL BIOLOGY, 1990, 6 :715-760

← 1 2 3 4 5 →