Carbon selection for nitrogen degradation pathway by Stenotrophomonas maltophilia: Based on the balances of nitrogen, carbon and electron

A novel strain DQ01 capable of simultaneous removal of nitrate and ammonium under the aerobic condition was isolated from the landfill leachate and identified as Stenotrophomonas maltophilia. The result showed that S. maltophilia had carbon selection for the nitrogen removal pathway, and preferred to utilize carboxylate rather than carbohydrate, as carboxylate could directly participate in TCA cycle without Embden Meyerhof Parmas (EMP). Nitrogen and carbon balances confirmed that the ammonium assimilation was the main or even sole removal pathway for S. maltophilia, and carboxylate was more conducive to heterotrophic nitrification-aerobic denitrification (HN-AD) process due to the serious self-alkalization and higher reduction potential of carboxylate, which followed: NH4+ -> NO2- -> NO3- -> NO2- -> NO due to the lack of nor and nos. Meanwhile, the higher C/N and nitrate could generate a more powerful ion transport driving force to accelerate the electron transfer in the denitrifying respiratory chain.