RNA-based gene transfer for adult stem cells and T cells

被引:51
作者
Smits, E [1 ]
Ponsaerts, P [1 ]
Lenjou, M [1 ]
Nijs, G [1 ]
Van Bockstaele, DR [1 ]
Berneman, ZN [1 ]
Van Tendeloo, VFI [1 ]
机构
[1] Univ Antwerp, Univ Antwerp Hosp, Fac Med, Lab Expt Hematol, B-2650 Edegem, Belgium
关键词
stem cells; T cells; electroporation; mRNA; EGFP;
D O I
10.1038/sj.leu.2403463
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Electroporation of mRNA has become an established method for gene transfer into dendritic cells for immunotherapeutic purposes. However, many more cell types and applications might benefit from an efficient mRNA-based gene transfer method. In this study, we investigated the potential of mRNA-based gene transfer to induce short-term transgene expression in adult stem cells and activated T cells, based on electroporation with mRNA encoding the enhanced green fluorescent protein. The results show efficient transgene expression in CD34-positive hematopoietic progenitor cells (35%), in in vitro cultured mesenchymal cells (90%) and in PHA-stimulated T cells (50%). Next to presentation of gene transfer results, potential applications of mRNA-based gene transfer in stem cells and T cells are discussed.
引用
收藏
页码:1898 / 1902
页数:5
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