PyNPase expression in human colon cancer

被引：14

作者：

Haba, A ^{[1
]}

Monden, T ^{[1
]}

Sekimoto, M ^{[1
]}

Ikeda, K ^{[1
]}

Izawa, H ^{[1
]}

Kanou, T ^{[1
]}

Amano, M ^{[1
]}

Kan'yama, H ^{[1
]}

Monden, M ^{[1
]}

机构：

[1] Osaka Univ, Sch Med, Dept Surg 2, Suita, Osaka 565, Japan

来源：

CANCER LETTERS | 1998年 / 122卷 / 1-2期

关键词：

PyNPase; colorectal cancer; stroma; protein extraction; western blot analysis;

D O I：

10.1016/S0304-3835(97)00375-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Although colorectal cancer tissue is rich in pyrimidine nucleoside phosphorylase (PyNPase), there is no consensus as to whether cancer cells or stromal cells predominately express PyNPase. We micro-dissected OCT compound embedded frozen tissue sections into epithelial and stromal components and then analyzed the extracted samples separately. The PyNPase expression level was higher in stromal cells than in cancer cells and the difference increased with inflammation induced by the immunostimulator OK432. These results suggest that stromal cells are the major PyNPase source in colorectal cancer. (C) 1998 Elsevier Science Ireland Ltd.

引用

页码：85 / 92

页数：8

共 15 条

[1] METABOLIC BREAKDOWN OF [H-3] THYMIDINE AND THE INABILITY TO MEASURE HUMAN-LYMPHOCYTE PROLIFERATION BY INCORPORATION OF RADIOACTIVITY [J].

BODYCOTE, J ;

WOLFF, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (13) :4749-4753

[2] ACTIVATION OF HUMAN PERIPHERAL BLOOD-DERIVED MONOCYTES BY OK-432 (STREPTOCOCCUS, PYOGENES) - AUGMENTED CYTO-TOXICITY AND SECRETION OF TNF AND SYNERGY WITH RIFN-GAMMA [J].

BONAVIDA, B ;

JEWETT, A .

CELLULAR IMMUNOLOGY, 1989, 123 (02) :373-383

[3] CYTOKINES INDUCE THYMIDINE PHOSPHORYLASE EXPRESSION IN TUMOR-CELLS AND MAKE THEM MORE SUSCEPTIBLE TO 5'-DEOXY-5-FLUOROURIDINE [J].

EDA, H ;

FUJIMOTO, K ;

WATANABE, S ;

URA, M ;

HINO, A ;

TANAKA, Y ;

WADA, K ;

ISHITSUKA, H .

CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1993, 32 (05) :333-338

[4] PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR THYMIDINE PHOSPHORYLASE EXPRESSION IN NORMAL-TISSUES - AN IMMUNOHISTOCHEMICAL STUDY [J].

FOX, SB ;

MOGHADDAM, A ;

WESTWOOD, M ;

TURLEY, H ;

BICKNELL, R ;

GATTER, KC ;

HARRIS, AL .

JOURNAL OF PATHOLOGY, 1995, 176 (02) :183-190

[5] PURINE NUCLEOSIDE SYNTHESIS, AN EFFICIENT METHOD EMPLOYING NUCLEOSIDE PHOSPHORYLASES [J].

KRENITSKY, TA ;

KOSZALKA, GW ;

TUTTLE, JV .

BIOCHEMISTRY, 1981, 20 (12) :3615-3621

[6]

MONDEN T, 1992, CANCER-AM CANCER SOC, V69, P636, DOI 10.1002/1097-0142(19920201)69:3<636::AID-CNCR2820690306>3.0.CO

[7]

2-W

[8]

Nishida M, 1996, BIOL PHARM BULL, V19, P1407

[9] MOLECULAR BIOLOGIC CONCEPTS OF CORONARY ANASTOMOSES [J].

SCHAPER, W ;

SHARMA, HS ;

QUINKLER, W ;

MARKERT, T ;

WUNSCH, M ;

SCHAPER, J .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1990, 15 (03) :513-518

[10]

TAGUCHI T, 1990, J IMMUNOL, V145, P68

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