Purification and identification of dipeptidyl peptidase (DPP) IV inhibitory peptides from the macroalga Palmaria palmata

被引：154
作者：
Harnedy, Padraigin A. [1 ]
O'Keeffe, Martina B. [1 ]
FitzGerald, Richard J. [1 ]
机构：
[1] Univ Limerick, Dept Life Sci, Limerick, Ireland
来源：
FOOD CHEMISTRY | 2015年 / 172卷
关键词：
Bioactive peptide; Dipeptidyl peptidase IV; Palmaria palmata; Type; 2; diabetes; PROTEIN; CASEIN; FRACTIONATION; ANTIOXIDANT; HYDROLYSATE; STABILITY; GLUCOSE;
D O I：
10.1016/j.foodchem.2014.09.083
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Dipeptidyl peptidase (DPP)-IV inhibitory peptides were purified and identified from an aqueous Palmaria palmata protein extract hydrolysed with Corolase PP. The hydrolysate was fractionated by solid phase extraction (SPE) using a C18 matrix followed by semi-preparative reverse phase-high performance liquid chromatography (SP RP-HPLC). IC50 values of 1.47 +/- 0.09, 0.54 +/- 0.03 and 0.36 +/- 0.03 mg/ml were obtained for the hydrolysate, the 25% - acetonitrile (ACN) SPE fraction and the most active SP RP-HPLC peptide fraction (SP RP-HPLC 25_F28), respectively. Thirteen peptide sequences were identified following UPLC-ESI MS/MS analysis of SP RP-HPLC 25_F28. Three novel DPP-IV inhibitory peptides, Ile-Leu-Ala-Pro, Leu-Leu-Ala-Pro and Met-Ala-Gly-Val-Asp-His-Ile, with IC50 values in the range 43-159 mu M were identified. The results indicate that P. palmata derived peptides may have potential as functional food ingredients in the prevention and management of type 2 diabetes. (C) 2014 Elsevier Ltd. All rights reserved.
引用
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页码：400 / 406
页数：7
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