Novel carbon black-cobalt phthalocyanine nanocomposite as sensing platform to detect organophosphorus pollutants at screen-printed electrode

被引：71
作者：
Cinti, S. [1 ,2 ]
Neagu, D. [1 ,2 ]
Carbone, M. [1 ,2 ]
Cacciotti, I. [3 ,4 ]
Moscone, D. [1 ,2 ]
Arduini, F. [1 ,2 ]
机构：
[1] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, Via Ric Sci, I-00133 Rome, Italy
[2] Consorzio Interuniv Biostrutture & Biosistemi INB, Viale Medaglie Oro 305, Rome, Italy
[3] Univ Roma Niccolo Cusano, Via Don Carlo Gnocchi 3, I-00166 Rome, Italy
[4] Consorzio Interuniv Nazl Sci & Tecnol Mat INSTM, Rome, Italy
来源：
ELECTROCHIMICA ACTA | 2016年 / 188卷
关键词：
Biosensor; Hybrid Nanocomposite; Carbon Black; Cobalt Phthalocyanine; Organophosphorus pesticide; ACETYLCHOLINESTERASE BIOSENSOR; ELECTROCATALYTIC ACTIVITY; MOLECULAR MATERIALS; NERVE AGENTS; NANOTUBES; NANOPARTICLES; PERFORMANCE; INHIBITION; PESTICIDES; SENSORS;
D O I：
10.1016/j.electacta.2015.11.069
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A facile "one-step" route to produce a homogenous and highly stable cobalt phthalocyanine (CoPc)-based dispersion by using carbon black (CB) as supporting material is reported. Herein, CB is proposed as effective material to load CoPc in order to obtain a CB/CoPc hybrid nanocomposite dispersion suitable for modifying screen-printed electrodes (SPEs) by an easy and automatable drop casting approach. CoPc resulted anchored to CB by a non-covalent physisorption, confirmed by IR and UV-visible spectroscopies, allowing to preserve the electrochemical performances of CoPc. The resulting CB/CoPc-modified SPE was tested as sensing tool to detect thiocholine, an enzymatic product of butyrylcholinesterase (BChE). The use of CB/CoPc leads to a highly sensitive thiocholine detection by applying a low potential (+0.05 V vs. internal reference) without fouling problem, a typical drawback that affects the thiol electrochemical detection. The favorable characteristics of the sensor were exploited for an easy BChE biosensor fabrication that renders this biosensor well suitable for mass-production. This electrochemical monoenzymatic biosensor was then challenged towards paraoxon, chosen as model organophosphorous pesticide, obtaining a low detection limit (18 nM). The suitability of the biosensor was tested in a waste water sample obtaining satisfactory recovery values, thus demonstrating its capability in such complex matrix. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：574 / 581
页数：8
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