Gene assembly based on blunt-ended double-stranded DNA-modules

被引：4

作者：

Dietrich, R ^{[1
]}

Wirsching, F ^{[1
]}

Opitz, T ^{[1
]}

Schwienhorst, A ^{[1
]}

机构：

[1] Inst Mol Biotechnol, Dept Mol Evolut Biol, D-07745 Jena, Germany

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 01期

关键词：

D O I：

10.1023/A:1008855526226

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene synthesis of leucine zipper genes based on blunt end ligation of ds DNA modules was performed in several ligation steps on magnetic beads. A 3'-phosphate, which was removed by T4 polynucleotide kinase after each ligation step, was used to guarantee orientation and single addition of newly added modules. Not depending on sequence complementarity the method appears well suited for the generation of combinatorial libraries of heterologous molecules.

引用

页码：49 / 54

页数：6

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