Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity

被引:30
|
作者
Sun, Yujuan [1 ]
Lin, Yanfei [1 ]
Cao, Xueli [1 ]
Xiang, Lan [1 ]
Qi, Jianhua [1 ]
机构
[1] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
neuroprotection; anti-aging; anti-oxidative stress; Mytilidae; sterols; UTH1; SOD; Alzheimer's disease; TRAUMATIC BRAIN-INJURY; OXIDATIVE-STRESS; ALZHEIMERS-DISEASE; LIFE-SPAN; PATHWAY; YEAST; NEURODEGENERATION; CRINOSTEROL; RAPAMYCIN; SOD;
D O I
10.3390/ijms151221660
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.
引用
收藏
页码:21660 / 21673
页数:14
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