Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity

被引:30
|
作者
Sun, Yujuan [1 ]
Lin, Yanfei [1 ]
Cao, Xueli [1 ]
Xiang, Lan [1 ]
Qi, Jianhua [1 ]
机构
[1] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
neuroprotection; anti-aging; anti-oxidative stress; Mytilidae; sterols; UTH1; SOD; Alzheimer's disease; TRAUMATIC BRAIN-INJURY; OXIDATIVE-STRESS; ALZHEIMERS-DISEASE; LIFE-SPAN; PATHWAY; YEAST; NEURODEGENERATION; CRINOSTEROL; RAPAMYCIN; SOD;
D O I
10.3390/ijms151221660
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function.
引用
收藏
页码:21660 / 21673
页数:14
相关论文
共 50 条
  • [1] Application of Glutathione as Anti-Oxidative and Anti-Aging Drugs
    Homma, Takujiro
    Fujii, Junichi
    CURRENT DRUG METABOLISM, 2015, 16 (07) : 560 - 571
  • [2] Anti-Oxidative and Anti-Aging Effects of Ethanol Extract of the Officinal Breynia (Breynia vitis-idaea) In Vitro
    Shin, Chae Yun
    Jang, Jiwon
    Lee, Hwa Pyoung
    Park, Sang Hee
    Kry, Masphal
    Keo, Omaliss
    Lee, Byoung-Hee
    Choi, Wooram
    Lee, Sarah
    Cho, Jae Youl
    PLANTS-BASEL, 2023, 12 (05):
  • [3] Neuroprotective effects of inhaled lavender oil on scopolamine-induced dementia via anti-oxidative activities in rats
    Hancianu, Monica
    Cioanca, Oana
    Mihasan, Marius
    Hritcu, Lucian
    PHYTOMEDICINE, 2013, 20 (05) : 446 - 452
  • [4] Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice
    Kwon, Seung-Hwan
    Lee, Ha-Kyung
    Kim, Ji-Ah
    Hong, Sa-Ik
    Kim, Hyoung-Chun
    Jo, Tae-Hyung
    Park, Young-In
    Lee, Chong-Kil
    Kim, Yong-Bin
    Lee, Seok-Yong
    Jang, Choon-Gon
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2010, 649 (1-3) : 210 - 217
  • [5] Neuroprotective Effects of Melatonin on Experimental Allergic Encephalomyelitis Mice Via Anti-Oxidative Stress Activity
    Long, Ting
    Yang, Yuan
    Peng, Ling
    Li, Zuoxiao
    JOURNAL OF MOLECULAR NEUROSCIENCE, 2018, 64 (02) : 233 - 241
  • [6] Discovery of novel N-substituted carbazoles as neuroprotective agents with potent anti-oxidative activity
    Zhu, Daqian
    Chen, Meihui
    Li, Min
    Luo, Bingling
    Zhao, Yang
    Huang, Peng
    Xue, Fengtian
    Rapposelli, Simona
    Pi, Rongbiao
    Wen, Shijun
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2013, 68 : 81 - 88
  • [7] Neuroprotective Effect of Catalpol via Anti-Oxidative, Anti-Inflammatory, and Anti-Apoptotic Mechanisms
    Yang, Chunjing
    Shi, Zhengyuan
    You, Longtai
    Du, Yuanyuan
    Ni, Jian
    Yan, Dan
    FRONTIERS IN PHARMACOLOGY, 2020, 11
  • [8] Neuroprotective Effects of Melatonin on Experimental Allergic Encephalomyelitis Mice Via Anti-Oxidative Stress Activity
    Ting Long
    Yuan Yang
    Ling Peng
    Zuoxiao Li
    Journal of Molecular Neuroscience, 2018, 64 : 233 - 241
  • [9] Neuroprotective Effects of Sinomenine on Experimental Autoimmune Encephalomyelitis via Anti-Inflammatory and Nrf2-Dependent Anti-Oxidative Stress Activity
    Fan, Hua
    Yang, Yang
    Bai, Qianqian
    Wang, Dongmei
    Shi, Xiaofei
    Zhang, Lele
    Yang, Yanhui
    NEUROMOLECULAR MEDICINE, 2023, 25 (04) : 545 - 562
  • [10] Anti-oxidative and Neuroprotective Activities of Pig Skin Gelatin Hydrolysates
    Kim, Dong Wook
    Park, Kimoon
    Ha, Goeun
    Jung, Ju Ri
    Chang, Ounki
    Ham, Jun-Sang
    Jeong, Seok-Geun
    Park, Beom-Young
    Song, Jin
    Jang, Aera
    KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, 2013, 33 (02) : 258 - 267