Oncogenic function of angiopoietin-2 in vitro and its modulation of tumor progression in colorectal carcinoma

被引：9

作者：

Kim, Hyungjoo ^{[1
]}

Ahn, Tae Sung ^{[2
]}

Kim, Chang-Jin ^{[3
]}

Bae, Sang Byung ^{[4
]}

Kim, Han Jo ^{[4
]}

Lee, Chang-Seuk ^{[5
]}

Kim, Tae Hyun ^{[5
]}

Im, Jungkyun ^{[6
]}

Lee, Sang Hun ^{[7
]}

Son, Myoung Won ^{[2
]}

Lee, Moon Soo ^{[2
]}

Baek, Moo Jun ^{[2
]}

Jeong, Dongjun ^{[3
]}

机构：

[1] Soonchunhyang Univ, Coll Med, Soonchunhyang Med Sci Res Inst, Cheonan 330721, Chungcheongnam, South Korea

[2] Soonchunhyang Univ, Coll Med, Dept Surg, Cheonan 330721, Chungcheongnam, South Korea

[3] Soonchunhyang Univ, Coll Med, Dept Pathol, 31 Soonchunhyang 6 Gil, Cheonan 330721, Chungcheongnam, South Korea

[4] Soonchunhyang Univ, Coll Med, Dept Pathol, Cheonan 330721, Chungcheongnam, South Korea

[5] Soonchunhyang Univ, Dept Chem, Asan 336745, Chungcheongnam, South Korea

[6] Soonchunhyang Univ, Dept Nanochem Engn, Asan 336745, Chungcheongnam, South Korea

[7] Soonchunhyang Univ, Dept Biochem, Coll Med, Cheonan 330721, Chungcheongnam, South Korea

来源：

ONCOLOGY LETTERS | 2017年 / 14卷 / 01期

关键词：

Angiopoietin-2; Colorectal carcinoma; shRNA; Oncogenic function; ENDOTHELIAL GROWTH-FACTOR; CLINICAL-SIGNIFICANCE; BREAST-CANCER; EXPRESSION; ANGIOGENESIS; THERAPY; SITE;

D O I：

10.3892/ol.2017.6203

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Angiopoietin-2 (Ang-2) has been investigated in cancer primarily in terms of its angiogenic function, and its role as an oncogene has yet to be elucidated. The current study hypothesized that Ang-2 may be an oncogene and have a function in tumor progression. An investigation of the function of Ang-2 in the LoVo colorectal cancer (CRC) cell line in vitro; which expresses a high level of Ang-2, was performed by knocking down endogenous expression with a targeted short hairpin RNA. The aggressive phenotypic effects of Ang-2 on experimental and control group cells were assessed using cell proliferation, migration and invasion assays. The association between Ang-2 expression levels and clinicopathological factors was evaluated in 415 CRC tissues using immunohistochemistry. Suppressing Ang-2 expression decreased cellular proliferation, invasion and migration in an in vitro study. Ang-2 overexpression was observed in 46% of patients with CRC and was significantly associated with pT (P=0.048), pN (P<0.001), venous invasion (P=0.023), lymphatic invasion (P<0.001) and tumor-node-metastasis stage (P=0.022). Furthermore, Ang-2 overexpression was an independent prognostic factor in pN stages 1 and 2. These results reveal that Ang-2 may be an oncogene in colorectal carcinogenesis and its expression may exert aggressive phenotypic effects during tumor progression. In addition, Ang-2 expression may serve as a prognostic marker and a potential drug target.

引用

页码：553 / 560

页数：8