Isolation and Characterization of Psacotheasin, a Novel Knottin-Type Antimicrobial Peptide, from Psacothea hilaris

被引：27
|
作者：
Hwang, Jae-Sam [2 ]
Lee, Juneyoung [1 ]
Hwang, Bomi [1 ]
Nam, Sung-Hee [2 ]
Yun, Eun-Young [2 ]
Kim, Seong-Ryul [2 ]
Lee, Dong Gun [1 ]
机构：
[1] Kyungpook Natl Univ, Coll Nat Sci, Sch Life Sci & Biotechnol, Taegu 702701, South Korea
[2] Natl Acad Agr Sci, Dept Agr Biol, RDA, Suwon 441100, South Korea
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 20卷 / 04期
关键词：
Psacothea hilaris; psacotheasin; knottin-type peptide; antimicrobial peptide; ANTIBACTERIAL; INSECTS;
D O I：
10.4014/jmb.1002.02003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI were 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.
引用
收藏
页码：708 / 711
页数：4
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