Gold nanoparticle-based signal augmentation of quartz crystal microbalance immunosensor measuring C-reactive protein

被引：34

作者：

Kim, N. ^{[1
]}

Kim, D. -K. ^{[1
]}

Cho, Y. -J. ^{[1
]}

机构：

[1] Korea Food Res Inst, Food Bionano Technol Res Grp, Songnam 463746, South Korea

来源：

CURRENT APPLIED PHYSICS | 2010年 / 10卷 / 04期

关键词：

Gold nanoparticle; Signal augmentation; Quartz crystal microbalance immunosensor; C-reactive protein; ANTIBODY IMMOBILIZATION; GRATING COUPLER; QUANTUM DOTS; SENSITIVITY; BIOSENSOR; SILICA; ELECTROCHEMILUMINESCENCE; LABELS;

D O I：

10.1016/j.cap.2010.02.048

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A biotinylated anti-rat C-reactive protein (CRP) antibody was ingeniously prepared by the reaction of the unmodified antibody with a water-soluble sulfosuccinimidyl-6-(biotinamido) hexanoate. The molar biotin incorporation of the resulting modified antibody was found as 5.82. A flow-type indirect-competitive quartz crystal microbalance immunosensor system was constructed with the sensor chip immobilized with 2 mg/mL CRP as the coating antigen. When 200 mu L of the modified antibody having the concentration of 0.250 mg/mL was added with a streptavidin-coated gold nanoparticle (GNP) to the immunosensor system, the frequency shift obtained was 139.8 +/- 0.3 Hz. Compared to the frequency shift of 91.1 +/- 1.3 Hz found with the addition of the unmodified antibody only, the signal augmentation after GNP binding amounted to 53.4%, which resulted in sensitivity improvement of the current immunosensor. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1227 / 1230

页数：4

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