Precise Two-Photon Photodynamic Therapy using an Efficient Photosensitizer with Aggregation-Induced Emission Characteristics

被引:283
作者
Gu, Bobo [1 ]
Wu, Wenbo [2 ]
Xu, Gaixia [3 ]
Feng, Guangxue [2 ]
Yin, Feng [4 ]
Chong, Peter Han Joo [5 ]
Qu, Junle [3 ]
Yong, Ken-Tye [1 ]
Liu, Bin [2 ]
机构
[1] Nanyang Technol Univ, Sch Elect & Elect Engn, 50 Nanyang Ave, Singapore 639798, Singapore
[2] Natl Univ Singapore, Dept Chem & Biomol Engn, 4 Sci Dr 4, Singapore 117585, Singapore
[3] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China
[4] Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Shenzhen 518055, Peoples R China
[5] Auckland Univ Technol, Dept Elect & Elect Engn, Auckland 92006, New Zealand
基金
中国国家自然科学基金; 新加坡国家研究基金会;
关键词
aggregation-induced emission; brain-blood-vessel closure; cancer cells; photodynamic therapy; two-photon excitation; FLUORESCENT ORGANIC DOTS; IN-VITRO; CELL; NANOPARTICLES; ABSORPTION; DESIGN; CANCER;
D O I
10.1002/adma.201701076
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Two-photon photodynamic therapy (PDT) is able to offer precise 3D manipulation of treatment volumes, providing a target level that is unattainable with current therapeutic techniques. The advancement of this technique is greatly hampered by the availability of photosensitizers with large two-photon absorption (TPA) cross section, high reactive-oxygen-species (ROS) generation efficiency, and bright two-photon fluorescence. Here, an effective photosensitizer with aggregation-induced emission (AIE) characteristics is synthesized, characterized, and encapsulated into an amphiphilic block copolymer to form organic dots for two-photon PDT applications. The AIE dots possess large TPA cross section, high ROS generation efficiency, and excellent photostability and biocompatibility, which overcomes the limitations of many conventional two-photon photosensitizers. Outstanding therapeutic performance of the AIE dots in two-photon PDT is demonstrated using in vitro cancer cell ablation and in vivo brain-blood-vessel closure as examples. This shows therapy precision up to 5 mu m under two-photon excitation.
引用
收藏
页数:7
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