Precise Two-Photon Photodynamic Therapy using an Efficient Photosensitizer with Aggregation-Induced Emission Characteristics

被引：283

作者：

Gu, Bobo ^{[1
]}

Wu, Wenbo ^{[2
]}

Xu, Gaixia ^{[3
]}

Feng, Guangxue ^{[2
]}

Yin, Feng ^{[4
]}

Chong, Peter Han Joo ^{[5
]}

Qu, Junle ^{[3
]}

Yong, Ken-Tye ^{[1
]}

Liu, Bin ^{[2
]}

机构：

[1] Nanyang Technol Univ, Sch Elect & Elect Engn, 50 Nanyang Ave, Singapore 639798, Singapore

[2] Natl Univ Singapore, Dept Chem & Biomol Engn, 4 Sci Dr 4, Singapore 117585, Singapore

[3] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China

[4] Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Shenzhen 518055, Peoples R China

[5] Auckland Univ Technol, Dept Elect & Elect Engn, Auckland 92006, New Zealand

来源：

ADVANCED MATERIALS | 2017年 / 29卷 / 28期

基金：

中国国家自然科学基金; 新加坡国家研究基金会;

关键词：

aggregation-induced emission; brain-blood-vessel closure; cancer cells; photodynamic therapy; two-photon excitation; FLUORESCENT ORGANIC DOTS; IN-VITRO; CELL; NANOPARTICLES; ABSORPTION; DESIGN; CANCER;

D O I：

10.1002/adma.201701076

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two-photon photodynamic therapy (PDT) is able to offer precise 3D manipulation of treatment volumes, providing a target level that is unattainable with current therapeutic techniques. The advancement of this technique is greatly hampered by the availability of photosensitizers with large two-photon absorption (TPA) cross section, high reactive-oxygen-species (ROS) generation efficiency, and bright two-photon fluorescence. Here, an effective photosensitizer with aggregation-induced emission (AIE) characteristics is synthesized, characterized, and encapsulated into an amphiphilic block copolymer to form organic dots for two-photon PDT applications. The AIE dots possess large TPA cross section, high ROS generation efficiency, and excellent photostability and biocompatibility, which overcomes the limitations of many conventional two-photon photosensitizers. Outstanding therapeutic performance of the AIE dots in two-photon PDT is demonstrated using in vitro cancer cell ablation and in vivo brain-blood-vessel closure as examples. This shows therapy precision up to 5 mu m under two-photon excitation.

引用

页数：7

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