Novel Utilization of Serum in Tissue Decellularization

被引：0
作者：
Gui, Liqiong [1 ,2 ]
Chan, Stephen A. [2 ,3 ]
Breuer, Christopher K. [2 ,4 ]
Niklason, Laura E. [1 ,2 ,3 ]
机构：
[1] Yale Univ, Dept Anesthesiol, Sch Med, New Haven, CT 06520 USA
[2] Yale Univ, Vasc Biol & Therapeut Program, Sch Med, New Haven, CT 06520 USA
[3] Yale Univ, Dept Biomed Engn, New Haven, CT 06520 USA
[4] Yale Univ, Dept Surg, Sch Med, New Haven, CT 06520 USA
来源：
TISSUE ENGINEERING PART C-METHODS | 2010年 / 16卷 / 02期
关键词：
AORTIC-VALVE ALLOGRAFTS; EXTRACELLULAR-MATRIX; IMMUNE-RESPONSE; BLOOD-VESSELS; VASCULAR GRAFTS; PORCINE VALVES; HEART-VALVES; SCAFFOLD; CELLS; ARTERIES;
D O I：
10.1089/ten.tec.2009.0120
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Decellularization of native tissues is a promising technique with numerous applications in tissue engineering and regenerative medicine. However, there are various limitations of currently available decellularization methods, such as alteration of extracellular matrix mechanics and restricted use on certain tissues. This study was conducted to explore the effect of serum on the decellularization of various types of tissues. Fetal bovine serum-containing cell culture medium endothelial growth media-2 removed DNA but not cellular beta-actin from human umbilical artery after detergent treatment, without compromising the tissue mechanical strength assessed by burst pressure. In addition, the effect of serum-containing endothelial growth media-2 on DNA removal was replicated in other types of tissues such as tissue-engineered vessels and myocardium. Other types of serum, including human serum, were also shown to remove DNA from detergent-pretreated tissues. In conclusion, we describe a novel utilization of serum that may have broad applications in tissue decellularization.
引用
收藏
页码：173 / 184
页数：12
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