Random expression of human immunodeficiency virus-1 (HIV-1) p17 (epitopes) on the surface of the HIV-1-infected cell

被引：13

作者：

Ota, A

Liu, XL

Fujio, H

Sakato, N

Ueda, S

机构：

[1] Osaka Univ, Microbial Dis Res Inst, Dept Neurovirol, Div Virol & Immunol, Suita, Osaka 565, Japan

[2] Chinese Acad Prevent Med, Ctr AIDS Surveillance, Beijing 100050, Peoples R China

[3] Kinki Sch Allied & Hlth Profess, Kita Ku, Osaka 531, Japan

[4] Kagawa Univ, Fac Agr, Dept Biosource Sci, Kagawa 76107, Japan

来源：

HYBRIDOMA | 1998年 / 17卷 / 01期

关键词：

D O I：

10.1089/hyb.1998.17.73

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Twenty monoclonal antibodies (MAbs) were obtained by immunizing Balb/c mice with recombinant p17 (rp17) of HIV-1, Epitope specificity of each MAb was determined using six peptides that cover the entire region of p17, We found that each MAb reacts with only one of the peptides, residues 12-29, 30-52, 53-87, and 87-115 (P12-29, P30-52, P53-87, P87-115) of p17 with the exception of one MAb, Three kinds of MAbs that recognize P30-52, P87-115, and a conformational epitope, suppressed the infectivity of HIV-1 (JMH-1) when they added in the culture of MT-4 cells infected by HIV-1 within 24 h of the infection.

引用

页码：73 / 75

页数：3

共 6 条

[1] PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].