In vitro anti-Candida activity of selective serotonin reuptake inhibitors against fluconazole-resistant strains and their activity against biofilm-forming isolates

被引：43

作者：

Costa Silva, Rose Anny ^{[1
,2
]}

da Silva, Cecilia Rocha ^{[1
,3
]}

de Andrade Neto, Joao Batista ^{[1
,2
]}

da Silva, Anderson Ramos ^{[1
]}

Campos, Rosana Sousa ^{[1
,3
]}

Sampaio, Leticia Serpa ^{[1
,2
]}

Stefany Aires do Nascimento, Francisca Bruna ^{[1
,2
]}

Gaspar, Brenda da Silva ^{[1
]}

da Cruz Fonseca, Said Goncalves ^{[1
]}

Alexandre Josino, Maria Aparecida ^{[1
,2
]}

Grangeiro, Thalles Barbosa ^{[5
]}

Gaspar, Danielle Macedo ^{[4
]}

de Lucena, David Freitas ^{[4
]}

de Moraes, Manoel Odorico ^{[4
]}

Cavalcanti, Bruno Coelho ^{[4
]}

Nobre Junior, Helio Vitoriano ^{[1
,2
]}

机构：

[1] Univ Fed Ceara, Dept Clin & Toxicol Anal, Sch Pharm, Lab Bioprospect Antimicrobial Mol LABIMAN, Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Sch Med, Dept Pathol & Legal Med, Fortaleza, Ceara, Brazil

[3] Christus Univ Ctr UNICHRISTUS, Fortaleza, Ceara, Brazil

[4] Univ Fed Ceara, Neuropharmacol Lab, Dept Physiol & Pharmacol, Fortaleza, Ceara, Brazil

[5] Univ Fed Ceara, Mol Genet Lab, Sci Ctr, Dept Biol, Fortaleza, Ceara, Brazil

来源：

MICROBIAL PATHOGENESIS | 2017年 / 107卷

关键词：

Candida spp; Biofilm; Fluoxetine; Paroxetine; Sertraline; ANTIFUNGAL ACTIVITY; EMERGING PATHOGENS; CANDIDA; FLUOXETINE; SERTRALINE; ANTIDEPRESSANTS; SUSCEPTIBILITY; EPIDEMIOLOGY; INDUCTION; APOPTOSIS;

D O I：

10.1016/j.micpath.2017.04.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recent research has shown broad antifungal activity of the classic antidepressants selective serotonin reuptake inhibitors (SSRIs). This fact, combined with the increased cross-resistance frequency of the genre Candida regarding the main treatment today, fluconazole, requires the development of novel therapeutic strategies. In that context, this study aimed to assess the antifungal potential of fluoxetine, sertraline, and paroxetine against fluconazole-resistant Candida spp. planktonic cells, as well as to assess the mechanism of action and the viability of biofilms treated with fluoxetine. After 24 h, the fluconazole-resistant Candida spp. strains showed minimum inhibitory concentration (MIC) in the ranges of 20 -160 mu g/mL for fluoxetine, 10-20 mu g/mL for sertraline, and 10-100.8 mu g/mL for paroxetine by the broth microdilution method (M27-A3). According to our data by flow cytometry, each of the SSRIs cause fungal death after damaging the plasma and mitochondrial membrane, which activates apoptotic signaling pathways and leads to dose-dependant cell viability loss. Regarding biofilm-forming isolates, the fluoxetine reduce mature biofilm of all the species tested. Therefore, it is concluded that SSRIs are capable of inhibit the growth in vitro of Candida spp., both in planktonic form, as biofilm, inducing cellular death by apoptosis. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：341 / 348

页数：8

共 39 条

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