Argonautes ALG-3 and ALG-4 are required for spermatogenesis-specific 26G-RNAs and thermotolerant sperm in Caenorhabditis elegans

被引：162

作者：

Conine, Colin C. ^{[1
]}

Batista, Pedro J. ^{[1
,3
]}

Gu, Weifeng ^{[1
]}

Claycomb, Julie M. ^{[1
,2
]}

Chaves, Daniel A. ^{[1
,4
]}

Shirayama, Masaki ^{[1
]}

Mello, Craig C. ^{[1
,2
]}

机构：

[1] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01606 USA

[2] Univ Massachusetts, Sch Med, Howard Hughes Med Inst, Worcester, MA 01606 USA

[3] Gulbenkian PhD Programme Biomed, P-2780156 Oeiras, Portugal

[4] Univ Lisbon, Fac Med, Inst Mol Med, P-1649028 Lisbon, Portugal

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 08期

关键词：

gene expression; RNAi; germline; P granules; spermiogenesis; C-ELEGANS; GENE-EXPRESSION; FERTILITY; 21U-RNAS; REVEALS; PIWI; MICRORNAS; GERMLINE; GRANULES; PATHWAY;

D O I：

10.1073/pnas.0911685107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gametogenesis is a thermosensitive process in numerous metazoans, ranging from worms to man. In Caenorhabditis elegans, a variety of RNA-binding proteins that associate with germ-line nuage (P granules), including the Piwi-clade argonaute PRG-1, have been implicated in maintaining fertility at elevated temperature. Here we describethe role of two AGO-class paralogs, alg-3(T22B3.2) and alg-4 (ZK757.3), in promoting thermotolerant male fertility. A rescuing GFP:: alg-3 transgene is localized to P granules beginning at the late pachytene stage of male gametogenesis. alg-3/4 double mutants lack a subgroup of small RNAs, the 26G-RNAs which target and appear to down-regulate numerous spermatogenesis-expressed mRNAs. These findings add to a growing number of AGO pathways required for thermotolerant fertility in C. elegans and support a model in which AGOs and their small RNA cofactors function to promote robustness in gene-expression networks.

引用

页码：3588 / 3593

页数：6

共 32 条

[1] RNA granules: post-transcriptional and epigenetic modulators of gene expression [J].

Anderson, Paul ;

Kedersha, Nancy .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (06) :430-436

[2] The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race [J].

Aravin, Alexei A. ;

Hannon, Gregory J. ;

Brennecke, Julius .

SCIENCE, 2007, 318 (5851) :761-764

[3] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[4] PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C-elegans [J].

Batista, Pedro J. ;

Ruby, J. Graham ;

Claycomb, Julie M. ;

Chiang, Rosaria ;

Fahlgren, Noah ;

Kasschau, Kristin D. ;

Chaves, Daniel A. ;

Gu, Weifeng ;

Vasale, Jessica J. ;

Duan, Shenghua ;

Conte, Darryl, Jr. ;

Luo, Shujun ;

Schroth, Gary P. ;

Carrington, James C. ;

Bartel, David P. ;

Mello, Craig C. .

MOLECULAR CELL, 2008, 31 (01) :67-78

[5]

BRENNER S, 1974, GENETICS, V77, P71

[6] MicroRNA functions [J].

Bushati, Natascha ;

Cohen, Stephen M. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :175-205

[7] The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation [J].

Claycomb, Julie M. ;

Batista, Pedro J. ;

Pang, Ka Ming ;

Gu, Weifeng ;

Vasale, Jessica J. ;

van Wolfswinkel, Josien C. ;

Chaves, Daniel A. ;

Shirayama, Masaki ;

Mitani, Shohei ;

Ketting, Rene F. ;

Conte, Darryl, Jr. ;

Mello, Craig C. .

CELL, 2009, 139 (01) :123-134

[8] Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline [J].

Das, Partha P. ;

Bagijn, Marloes P. ;

Goldstein, Leonard D. ;

Woolford, Julie R. ;

Lehrbach, Nicolas J. ;

Sapetschnig, Alexandra ;

Buhecha, Heeran R. ;

Gilchrist, Michael J. ;

Howe, Kevin L. ;

Stark, Rory ;

Matthewss, Nik ;

Berezilkov, Eugene ;

Ketting, Ren F. ;

Tavare, Simon ;

Miska, Eric A. .

MOLECULAR CELL, 2008, 31 (01) :79-90

[9]

Duchaine TF, 2006, CELL, V124, P343, DOI [10.1016/j.cell.2005.11.036, 10.1016/j.cel.2005.11.036]

[10] Single-copy insertion of transgenes in Caenorhabditis elegans [J].

Frokjaer-Jensen, Christian ;

Davis, M. Wayne ;

Hopkins, Christopher E. ;

Newman, Blake J. ;

Thummel, Jason M. ;

Olesen, Soren-Peter ;

Grunnet, Morten ;

Jorgensen, Erik M. .

NATURE GENETICS, 2008, 40 (11) :1375-1383

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