Sequence-directed DNA translocation by purified FtsK

被引:152
|
作者
Pease, PJ
Levy, O
Cost, GJ
Gore, J
Ptacin, JL
Sherratt, D
Bustamante, C [1 ]
Cozzarelli, NR
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA
[4] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[5] Univ Oxford, Dept Biochem, Div Mol Genet, Oxford OX1 3QU, England
关键词
D O I
10.1126/science.1104885
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
DNA translocases are molecular motors that move rapidly along DNA using adenosine triphosphate as the source of energy. We directly observed the movement of purified FtsK, an Escherichia coli translocase, on single DNA molecules. The protein moves at 5 kilobases per second and against forces up to 60 piconewtons, and locally reverses direction without dissociation. On three natural substrates, independent of its initial binding position, FtsK efficiently translocates over long distances to the terminal region of the E. coli chromosome, as it does in vivo. Our results imply that FtsK is a bidirectional motor that changes direction in response to short, asymmetric directing DNA sequences.
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页码:586 / 590
页数:5
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