Genetic diversity and variation in wild populations of dark sleeper (Odontobutis potamophila) in China inferred with microsatellite markers

被引：5
作者：
Zhu, Fei [1 ,2 ]
Zhang, Li-Juan [1 ,3 ]
Yin, Shao-Wu [1 ,3 ]
Zhang, Hong-Wei [1 ,3 ]
Hou, Xin-Yuan [1 ,3 ]
Hu, Ya-Li [1 ,3 ]
Luo, Jian [1 ]
机构：
[1] Nanjing Normal Univ, Coll Life Sci, Key Lab Biodivers & Biotechnol Jiangsu Prov, Nanjing 210023, Jiangsu, Peoples R China
[2] Marine Fisheries Res Inst Jiangsu Prov, Nantong 226007, Peoples R China
[3] Coinnovat Ctr Marine Bioind Technol Jiangsu Prov, Lian Yungang 222005, Peoples R China
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2014年 / 57卷
关键词：
Odontobutis potamophila; Microsatellite markers; Genetic variation; SOFTWARE;
D O I：
10.1016/j.bse.2014.07.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Twenty-one highly variable microsatellite loci were used to investigate the genetic diversity and variation of Odontobutis potamophila in China. A total of 160 samples from five wild populations (Dangtu, Sheyang, Yuyao, Minjiang and Donxishan) were genotyped. All of the 21 microsatellite loci tested in this study showed polymorphism. The number of allele per locus ranged from 5.05 to 9.90. Locus 87a of Minjiang population had a 259-bp characteristic allele. The average observed heterozygosity and expected heterozygosity ranged from 0.33 to 0.62 and from 0.40 to 0.70, respectively. The pair-wise F-ST tests and NJ trees of the five O. potamophlia populations revealed that Dangtu, Sheyang, Yuyao and Dongxishan were genetically close to one another and distinct from Minjiang. Far genetic distances were observed among populations from distant geographical areas. This result provided guide for the use of O. potamophila breeds and the protection of the species. (C) 2014 Elsevier Ltd. All rights reserved.
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页码：40 / 47
页数：8
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