Intraspecies variability of Desulfovibrio desulfuricans strains determined by the genetic profiles

被引：11

作者：

Dzierzewicz, Z ^{[1
]}

Szczerba, J ^{[1
]}

Weglarz, L ^{[1
]}

Swiatkowska, L ^{[1
]}

Jasinska, D ^{[1
]}

Wilczok, T ^{[1
]}

机构：

[1] Med Univ Silesia, Dept Mol Biol Biochem & Biopharm, PL-41200 Sosnowiec, Poland

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 219卷 / 01期

关键词：

repetitive extragenic palindromic; enterobacterial repetitive intergenic consensus; sulfate-reducing bacteria; DNA fingerprinting; Desulfovibrio desulfuricans;

D O I：

10.1016/S0378-1097(02)01199-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fifteen (soil and intestinal) strains of Desulfovibrio desulfuricans species were typed by PCR method with the use of primers specific for repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) sequences. As a result, characteristic DNA fingerprints for the strains were obtained. Moreover, the genetic profiles were found to be useful for typing and distinguishing the strains of D. desulfuricans. According to cluster analysis, PCR with primers complementary to the sequences REP appeared to be slightly more discriminatory than PCR with ERIC primers for the investigated strains. Distinct fingerprint patterns of two isolates derived from the same patient pointed to the different origin of both strains. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：69 / 74

页数：6

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