Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene

被引：27
作者：
Chen, Z
Zeng, H
Gorczynski, RM
机构：
[1] Univ Toronto, Toronto Hosp, Dept Surg, Transplant Res Div, Toronto, ON M5G 2C4, Canada
[2] Univ Toronto, Toronto Hosp, Dept Immunol, Transplant Res Div, Toronto, ON M5G 2C4, Canada
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1997年 / 1362卷 / 01期
关键词：
MRC OX-2; tolerance; immunoregulation; transplantation;
D O I：
10.1016/S0925-4439(97)00058-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 350bp amplicon, obtained by PCR-select subtractive hybridization from RNA derived from mesenteric lymph nodes (MLN) cells from mice pre-immunized with allogeneic lymphocytes 36hrs prior to receiving donor-specific skin grafts, and showing >98% homology with a published sequence for the rat MRC OX-2 gene, was used as a hybridization probe to screen a cDNA library constructed from adult mouse MLN treated in the same fashion. Several clones were identified which, on DNA sequence analysis, predicted a 218 amino acid protein showing significant homology with the rat and human MRC OX-2 gene product. (C) 1997 Elsevier Science B.V.
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页码：6 / 10
页数：5
相关论文
共 22 条
[1]  
ADKINS B, 1994, J IMMUNOL, V153, P3378
[2]   T-cell-receptor affinity and thymocyte positive selection [J].
Alam, SM ;
Travers, PJ ;
Wung, JL ;
Nasholds, W ;
Redpath, S ;
Jameson, SC ;
Gascoigne, NRJ .
NATURE, 1996, 381 (6583) :616-620
[3]  
BARCLAY AN, 1981, IMMUNOLOGY, V44, P727
[4]   PURIFICATION AND CHEMICAL CHARACTERIZATION OF MEMBRANE-GLYCOPROTEINS FROM RAT THYMOCYTES AND BRAIN, RECOGNIZED BY MONOCLONAL-ANTIBODY MRC-OX2 [J].
BARCLAY, AN ;
WARD, HA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 129 (02) :447-458
[5]   CLONING, MAPPING, AND CHARACTERIZATION OF ACTIVATED LEUKOCYTE-CELL ADHESION MOLECULE (ALCAM), A CD6 LIGAND [J].
BOWEN, MA ;
PATEL, DD ;
LI, X ;
MODRELL, B ;
MALACKO, AR ;
WANG, WC ;
MARQUARDT, H ;
NEUBAUER, M ;
PESANDO, JM ;
FRANCKE, U ;
HAYNES, BF ;
ARUFFO, A .
JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 181 (06) :2213-2220
[6]   MRC OX-2 ANTIGEN - A LYMPHOID NEURONAL MEMBRANE GLYCOPROTEIN WITH A STRUCTURE LIKE A SINGLE IMMUNOGLOBULIN LIGHT CHAIN [J].
CLARK, MJ ;
GAGNON, J ;
WILLIAMS, AF ;
BARCLAY, AN .
EMBO JOURNAL, 1985, 4 (01) :113-118
[7]   MECHANISMS OF LEUKOCYTE MOTILITY AND CHEMOTAXIS [J].
DOWNEY, GP .
CURRENT OPINION IN IMMUNOLOGY, 1994, 6 (01) :113-124
[8]  
GANGEMI RMR, 1989, J IMMUNOL, V143, P2439
[9]   Interleukin-13, in combination with anti interleukin-12, increases graft prolongation after portal venous immunization with cultured allogeneic bone marrow-derived dendritic cells [J].
Gorczynski, RM ;
Cohen, Z ;
Fu, XM ;
Hua, Z ;
Sun, YL ;
Chen, ZQ .
TRANSPLANTATION, 1996, 62 (11) :1592-1600
[10]   THE GENE FOR MRC OX-2 MEMBRANE GLYCOPROTEIN IS LOCALIZED ON HUMAN CHROMOSOME-3 [J].
MCCAUGHAN, GW ;
CLARK, MJ ;
HURST, J ;
GROSVELD, F ;
BARCLAY, AN .
IMMUNOGENETICS, 1987, 25 (02) :133-135
123