The roles of V-type H+-ATPase and Na+/K+-ATPase in energizing K+ and H+ transport in larval Drosophila gut epithelia

被引:18
作者
D'Silva, Natalie M. [1 ]
Donini, Andrew [2 ]
O'Donnell, Michael J. [1 ]
机构
[1] McMaster Univ, 1280 Main St W, Hamilton, ON L8S 4K1, Canada
[2] York Univ, 4700 Keele St, Toronto, ON M3J 1P3, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Drosophila melanogaster; V-type H+-ATPase; Na+/K+ ATPase; Ion transport; BASOLATERAL ION-TRANSPORT; MALPIGHIAN TUBULES; PROTON PUMP; FLUID SECRETION; MELANOGASTER; ACID; COTRANSPORT; MECHANISMS; MODEL; PH;
D O I
10.1016/j.jinsphys.2017.01.019
中图分类号
Q96 [昆虫学];
学科分类号
摘要
We analyzed V-type H+-ATPase (VA) and Na+/K+-ATPase (NKA) along the caeca and midgut of third instar Drosophila larvae using immunohistochemistry and ATPase activity assays. Corresponding H+ and K+ fluxes were characterized using the Scanning Ion-Selective Electrode Technique (SIET), and the roles of transport ATPases in energizing ion transport across the larval gut were investigated by basal application of bafilomycin, a VA inhibitor, and ouabain, a NKA inhibitor. Addition of bafilomycin led to a decrease in H+ absorption along the caeca and midgut except at the copper cells and large flat cell zone of the middle midgut. H+ absorption was decreased by acetazolamide, consistent with carbonic anhydrase activity in all regions except at the large flat cell zone of the middle midgut. Bafilomycin or acetazolamide also led to decreased K+ absorption across the caeca and the anterior midgut. Our data show the dependence of K+ transport on H+ gradients established by the VA in the latter regions, consistent with the presence of a Cation-Proton Antiporter (CPA2) identified in other insect epithelia. Addition of ouabain led to the increase of absorption along the anterior midgut and the large flat cell zone of the middle midgut, suggesting a role for the NKA in these regions. This study shows the importance of both ATPases in driving ion transport across the gut of larval Drosophila. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:284 / 290
页数:7
相关论文
共 50 条
[21]   Unexpected Effects of K+ and Adenosine Triphosphate on the Thermal Stability of Na+,K+-ATPase [J].
Agueda Placenti, M. ;
Kaufman, Sergio B. ;
Gonzalez Flecha, F. Luis ;
Gonzalez Lebrero, Rodolfo M. .
JOURNAL OF PHYSICAL CHEMISTRY B, 2017, 121 (19) :4949-4957
[22]   Involvement of Protons in the Ion Transport Cycle of Na+,K+-ATPase [J].
Grishanin, K. O. ;
Tashkin, V. Yu. ;
Lenz, A. A. ;
Apell, H. J. ;
Sokolov, V. S. .
BIOLOGICHESKIE MEMBRANY, 2010, 27 (06) :512-518
[23]   IMMUNOCYTOCHEMICAL LOCALIZATION OF NA+/K+-ATPASE AND V-H+-ATPASE IN THE SALIVARY-GLANDS OF THE COCKROACH, PERIPLANETA-AMERICANA [J].
JUST, F ;
WALZ, B .
CELL AND TISSUE RESEARCH, 1994, 278 (01) :161-170
[24]   Trophectoderm development and function:: the roles of Na+/K+-ATPase subunit isoforms [J].
Kidder, GM .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2002, 80 (02) :110-115
[25]   Transport of H+, Na+ and K+ across the posterior midgut of blood-fed mosquitoes (Aedes aegypti) [J].
Pacey, Evan K. ;
O'Donnell, Michael J. .
JOURNAL OF INSECT PHYSIOLOGY, 2014, 61 :42-50
[26]   V-H+-ATPase, Na+/K+-ATPase and NHE2 immunoreactivity in the gill epithelium of the Pacific hagfish (Epatretus stoutii) [J].
Tresguerres, Martin ;
Parks, Scott K. ;
Goss, Greg G. .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2006, 145 (03) :312-321
[27]   An in vitro analysis of intestinal ammonia transport in fasted and fed freshwater rainbow trout: roles of NKCC, K+ channels, and Na+, K+ ATPase [J].
Rubino, Julian G. ;
Wilson, Jonathan M. ;
Wood, Chris M. .
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 2019, 189 (05) :549-566
[28]   The V-type H+-ATPase in Malpighian tubules of Aedes aegypti:: localization and activity [J].
Weng, XH ;
Huss, M ;
Wieczorek, H ;
Beyenbach, KW .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2003, 206 (13) :2211-2219
[29]   The influence of potassium ion (K+) on digoxin-induced inhibition of porcine cerebral cortex Na+/K+-ATPase [J].
Krstic, Danijela ;
Tomic, Nenad ;
Krinulovic, Katarina ;
Vasic, Vesna .
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2006, 21 (04) :471-475
[30]   In vitro effect of different Na+/K+ ratios on plasma membrane H+-ATPase activity in maize and sugar beet shoot [J].
Wakeel, Abdul ;
Suemer, Ali ;
Hanstein, Stefan ;
Yan, Feng ;
Schubert, Sven .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2011, 49 (03) :341-345