N-3 polyunsaturated fatty acid DHA during IVM affected oocyte developmental competence in cattle

被引:47
作者
Oseikria, Mouhamad [1 ,2 ,3 ,4 ]
Elis, Sebastien [1 ,2 ,3 ,4 ]
Maillard, Virginie [1 ,2 ,3 ,4 ]
Corbin, Emilie [1 ,2 ,3 ,4 ]
Uzbekova, Svetlana [1 ,2 ,3 ,4 ]
机构
[1] INRA, Physiol Reprod & Comportements UMR85, F-37380 Nouzilly, France
[2] CNRS, UMR Physiol Reprod & Comportements 7247, Nouzilly, France
[3] Univ Francois Rabelais Tours, UMR Physiol Reprod & Comportements, Nouzilly, France
[4] IFCE, UMR Physiol Reprod & Comportements, Nouzilly, France
关键词
Oocyte competence; IVM; n-3; PUFA; Cumulus; Gene expression; LACTATING DAIRY-COWS; ALPHA-LINOLENIC ACID; CUMULUS CELLS; FOLLICULAR-FLUID; EMBRYO QUALITY; LIPID RAFTS; MATURATION; SUPPLEMENTATION; REPRODUCTION; FERTILITY;
D O I
10.1016/j.theriogenology.2016.01.019
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The positive effect of n-3 polyunsaturated fatty acids (FAs) on fertility in ruminants seems to be partly mediated through direct effects on the oocyte developmental potential. We aimed to investigate whether supplementation with physiological levels of docosahexaenoic acid (DHA, C22:6 n-3 polyunsaturated fatty acids) during IVM has an effect on oocyte maturation and in vitro embryo development in cattle. We reported that DHA (0, 1, 10, or 100 mu M) had no effect on oocyte viability or maturation rate after 22-hour IVM. Incubation of oocyte-cumulus complexes with 1-mu M DHA during IVM significantly increased (P < 0.05) oocyte cleavage rate as compared with control (86.1% vs. 78.8%, respectively) and the greater than 4-cell embryo rate at Day 2 after parthenogenetic activation (39.1% vs. 29.7%, respectively). Supplementation with 1 mu M DHA during IVM also induced a significant increase in the blastocyst rate at Day 7 after IVF as compared with control (30.6% vs. 17.6%, respectively) and tended to increase the number of cells in the blastocysts (97.1 +/- 4.9 vs. 81.2 +/- 5.3, respectively; P = 0.08). On the contrary, 10-mu M DHA had no effects, whereas 100-mu M DHA significantly decreased the cleavage rate compared with control (69.5% vs.78.8%, respectively) and the greater than 4-cell embryo rate at Day 2 after parthenogenetic activation (19.5% vs. 29.7%). As was shown by real-time polymerase chain reaction, negative effects of 100-mu M DHA were associated with significant increase of progesterone synthesis by oocyte-cumulus complexes, a three-fold increase in expression level of FA transporter CD36 and a two-fold decrease of FA synthase FASN genes in cumulus cells (CCs) of corresponding oocytes. Docosahexaenoic acid at 1 and 10 mu M had no effect on expression of those and other key lipid metabolism-related genes in CC. In conclusion, administration of a low physiological dose of DHA (1 mu M) during IVM may have beneficial effects on oocyte developmental competence in vitro without affecting lipid metabolism gene expression in surrounding CCs, contrarily to 100 mu M DHA which diminished oocyte quality associated with perturbation of lipid and steroid metabolism in CC. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:1625 / 1634
页数:10
相关论文
共 68 条
[1]   Bovine Cumulus Cells Protect Maturing Oocytes from Increased Fatty Acid Levels by Massive Intracellular Lipid Storage [J].
Aardema, Hilde ;
Lolicato, Francesca ;
van de Lest, Chris H. A. ;
Brouwers, Jos F. ;
Vaandrager, Arie B. ;
van Tol, Helena T. A. ;
Roelen, Bernard A. J. ;
Vos, Peter L. A. M. ;
Helms, J. Bernd ;
Gadella, Bart M. .
BIOLOGY OF REPRODUCTION, 2013, 88 (06)
[2]   Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte Developmental Competence [J].
Aardema, Hilde ;
Vos, Peter L. A. M. ;
Lolicato, Francesca ;
Roelen, Bernard A. J. ;
Knijn, Hiemke M. ;
Vaandrager, Arie B. ;
Helms, J. Bernd ;
Gadella, Bart M. .
BIOLOGY OF REPRODUCTION, 2011, 85 (01) :62-69
[3]   Effect of PUFA on embryo cryoresistance, gene expression and AMPKα phosphorylation in IVF-derived bovine embryos [J].
Al Darwich, Abdulrahman ;
Perreau, Christine ;
Petit, Marie Helene ;
Papillier, Pascal ;
Dupont, Joelle ;
Guillaume, Daniel ;
Mermillod, Pascal ;
Guignot, Florence .
PROSTAGLANDINS & OTHER LIPID MEDIATORS, 2010, 93 (1-2) :30-36
[4]   Lower pregnancy losses in lactating dairy cows fed a diet enriched in α-linolenic acid [J].
Ambrose, D. J. ;
Kastelic, J. P. ;
Corbett, R. ;
Pitney, P. A. ;
Petit, H. V. ;
Small, J. A. ;
Zalkovic, P. .
JOURNAL OF DAIRY SCIENCE, 2006, 89 (08) :3066-3074
[5]   Effects of polyunsaturated fatty acids from plant oils and algae on milk fat yield and composition are associated with mammary lipogenic and SREBF1 gene expression [J].
Angulo, J. ;
Mahecha, L. ;
Nuernberg, K. ;
Nuernberg, G. ;
Dannenberger, D. ;
Olivera, M. ;
Boutinaud, M. ;
Leroux, C. ;
Albrecht, E. ;
Bernard, L. .
ANIMAL, 2012, 6 (12) :1961-1972
[6]   Absence of cumulus cells during in vitro maturation affects lipid metabolism in bovine oocytes [J].
Auclair, Sylvain ;
Uzbekov, Rustem ;
Elis, Sebastien ;
Sanchez, Laura ;
Kireev, Igor ;
Lardic, Lionel ;
Dalbies-Tran, Rozenn ;
Uzbekova, Svetlana .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2013, 304 (06) :E599-E613
[7]   Metabolite concentrations in follicular fluid may explain differences in fertility between heifers and lactating cows [J].
Bender, K. ;
Walsh, S. ;
Evans, A. C. O. ;
Fair, T. ;
Brennan, L. .
REPRODUCTION, 2010, 139 (06) :1047-1055
[8]   Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in summer [J].
Bilby, T. R. ;
Block, J. ;
do Amaral, B. C. ;
Sa Filho, O. ;
Silvestre, F. T. ;
Hansen, P. J. ;
Staples, C. R. ;
Thatcher, W. W. .
JOURNAL OF DAIRY SCIENCE, 2006, 89 (10) :3891-3903
[9]   Alteration of energy metabolism gene expression in cumulus cells affects oocyte maturation via MOS-mitogen-activated protein kinase pathway in dairy cows with an unfavorable "Fertil-" haplotype of one female fertility quantitative trait locus [J].
Brisard, Daphne ;
Desmarchais, Alice ;
Touze, Jean-Luc ;
Lardic, Lionel ;
Freret, Sandrine ;
Elis, Sebastien ;
Nuttinck, Fabienne ;
Camous, Sylvaine ;
Dupont, Joelle ;
Uzbekova, Svetlana .
THERIOGENOLOGY, 2014, 81 (04) :599-612
[10]   Differential effects of n-3 and n-6 fatty acids on prostaglandin F2α production by bovine endometrial cells [J].
Caldari-Torres, C ;
Rodriguez-Sallaberry, C ;
Greene, ES ;
Badinga, L .
JOURNAL OF DAIRY SCIENCE, 2006, 89 (03) :971-977